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Leptomonas seymouri Wallace 拉丁名

(ATCC? 30220?) 統(tǒng)一編號(hào)

Biosafety Level 生物安全等級(jí) 1 

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. 

Isolation 分離基物 Dysdercus suturellus, Stuart, FL, 1959 

Product Format 提供形式 frozen 

Type Strain 模式菌株 no 

Comments 注釋 species description； 

Trypanosomatids from fruit； 

Distribution of carbohydrate epitopes；

Proteolytic activities； 

Acetylornithinase and ornithine acetyltransferase； 

isoenzyme electrophoresis for species identification； 

Cyclopropane fatty acid； 

enzymes of ornithine-arginine metabolism； 

Inhibition by antiprotozoal drugs； 

Cysteine and metalloproteinase activities； 

Monoclonal antibodies for identification； 

isoleucine requirement and threonine deaminase； conservation of 29-base-pair sequence within maxicircle ARS fragments； 

Medium 培養(yǎng)基 ATCC? Medium 1011: Diphasic blood agar medium 

Growth Conditions 生長(zhǎng)條件

Temperature 培養(yǎng)溫度: 25.0°C 

Duration: axenic 

Protocol: ATCC NO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly. 

Subcultivation Protocol: ATCC NO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.               

Name of Depositor 寄存人 FG Wallace 

Year of Origin 收藏年份 1959 

References 參考文獻(xiàn) Wallace FG. Leptomonas seymouri sp. n. from the cotton stainer Dysdercus suturellus. J. Protozool. 24: 483-484, 1977. PubMed: 599500 

Conchon I, et al. Trypanosomatids, other than Phytomonas spp., isolated and cultured from fruit. J. Protozool. 36: 412-414, 1989. 

Gazzinelli RT, et al. Distribution of carbohydrates recognized by the lectins Euonymus europaeus and concanavalin A in monoxenic and heteroxenic trypanosomatids. J. Protozool. 38: 320-325, 1991. PubMed: 1787421 

Camargo EP, et al. Proteolytic activities in cell extracts of trypanosomatids. J. Parasitol. 64: 1120-1121, 1978. PubMed: 739304 

Teixeira MM, Camargo EP. Monoclonal antibodies for the identification of trypanosomatids of the genus Phytomonas. J. Protozool. 36: 262-264, 1989. 

Teixeira MM, et al. Characterization of the target antigens of Phytomonas-specific monoclonal antibodies. J. Eukaryot. Microbiol. 42: 232-237, 1995. 

Branquinha MH, et al. Ubiquity of cysteine-and metalloproteinase activites in a wide range of trypanosomatids. J. Eukaryot. Microbiol. 43: 131-135, 1996. PubMed: 8720943 

Bacchi J, et al. Susceptibility of an insect Leptomonas and Crithidia fasciculata to several established antitrypanosomatid agents. Antimicrob. Agents Chemother. 6: 785-790, 1974. PubMed: 4451351 

Bacchi CJ, et al. Polyamines in trypanosomatids. J. Bacteriol. 131: 657-661, 1977. PubMed: 885842 

Galinari S, Camargo EP. Trypanosomatid protozoa: survey of acetylornithinase and ornithine acetyltransferase. Exp. Parasitol. 46: 277-282, 1978. PubMed: 569594 

Goncalves de Lima VM, et al. Five trypanosomatid species of insects distinguished by isoenzymes. J. Protozool. 26: 648-652, 1979. PubMed: 161788 

Fish WR, et al. The cyclopropane fatty acid of trypanosomatids. Mol. Biochem. Parasitol. 3: 103-115, 1981. PubMed: 7254247 

Kim R, Ray DS. Conservation of a 29-base-pair sequence within maxicircle ARS fragments from six species of trypanosomes. Gene 40: 291-299, 1985. PubMed: 3938423 

Camargo EP, et al. Trypanosoma spp., Leishmania spp. and Leptomonas spp.: enzymes of ornithine-arginine metabolism. Exp. Parasitol. 46: 141-144, 1978. PubMed: 569593 

Alfieri SC, Camargo EP. Trypanosomatidae: isoleucine requirement and threonine deaminase in species with and without endosymbionts. Exp. Parasitol. 53: 371-380, 1982. PubMed: 6806116 

Goldberg B, et al. Inhibition by several standard antiprotozoal drugs of growth and O2 uptake of cells and particulate preparations of a Leptomonas. J. Protozool. 21: 322-326, 1974. PubMed: 4365631 

Cho J, Eichinger D. Crithidia fasciculata induces encystation of Entamoeba invadens in a galactose-dependent manner. J. Parasitol. 84: 705-710, 1998. PubMed: 9714198 

Cross References Nucleotide (GenBank) : X07487 Leptomonas seymouri mini-exon donor RNA (medRNA). 

Nucleotide (GenBank) : X78552 L.seymouri (ATCC 30220) U6 snRNA, tRNA-Ile and tRNA-Gln genes.