ATCC? Number:30213?
Organism: Trypanosoma lewisi (Kent) Laveran and Mesnil
Designations: C (Costa Rica)
Isolation: rat, Rattus rattus, San Jose, Costa Rica, 1961
Depositors: CM Lee
History: ATCC<<--CM Lee<<--D. Lincicome
Biosafety Level:1
Shipped: frozen
Growth Conditions: Duration: in vivo, laboratory rat Protocol: ATCCNO: 30085 SPEC: Inject the entire thawed contents of the ampule intraperitoneally into an adult rat known to be free of blood parasites. The strain should be monitored, especially after infecting with thawed material. To infect additional rats, draw a drop of tail blood into a 1.0 ml tuberculin syringe containing 0.5 ml of sterile buffered anticoagulant, mix, and inoculate 0.1-0.2 ml of the suspension intraperitoneally/rat. Only a few trypanosomes are needed to infect a non-immune rat. However, do not wait until the blood is swarming with trypanosomes. The time for peak parasitemia may be within 4-5 days if large inocula are given. Peak parasitemia from the thawed material may be slightly sooner or later.
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Classification: KINGDOM: Protozoa
References: 4867: . . Parasitology 55: 365-373, 1965.