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平臺編號：bio-48617
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國際編號：ATCC 30176
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拉丁屬名： Naegleria fowleri
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規(guī)格：frozen
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用途：ATCC原裝進口
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服務費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產品信息以出庫為準）
HB3
ATCC? Number:30176?
Organism: Naegleria fowleri Carter
Designations: HB3
Isolation: human, Northern Bohemia, 1968
Depositors: CG Culbertson
History: ATCC<<--CG Culbertson<<--L. Cerva
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 1034: Modified PYNFH medium (Available from ATCCas ATCCcat. no. 327-X)ATCCmedium 722: Minimum essential medium (MEM)Temperature: 35.0°C Duration: grown with Macaca mulatta kidney cells, ATCCCCL 7.1 Protocol: ATCCNO: 30100 SPEC: Grown with Macaca mulatta kidney cells, ATCCCCL 7.1. Kidney cells for use with the amebae are grown in screw-capped tubes containing 2.0 ml of ATCCmedium 722. When tissue cultures are well established, inoculate with 0.1 ml from a flourishing amebae parent culture. Cultures must be gassed with 10% CO2 and 90% air after inoculation and at 4-day intervals. Incubate inoculated amebae cultures on a slant.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Can be cultivated in ATCCmedium 1034 without the cell line.
References: 4585: Cerva L, Novak K. Amoebic meningoencephalitis: 16 fatalities. Science 160: 92, 1968. PubMed: 5642317 5111: Cerva L, et al. An outbreak of acute, fatal amebic meningoencephalitis. Am. J. Epidemiol. 88: 436-444, 1968. PubMed: 5698435
Organism: Naegleria fowleri Carter
Designations: HB3
Isolation: human, Northern Bohemia, 1968
Depositors: CG Culbertson
History: ATCC<<--CG Culbertson<<--L. Cerva
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 1034: Modified PYNFH medium (Available from ATCCas ATCCcat. no. 327-X)ATCCmedium 722: Minimum essential medium (MEM)Temperature: 35.0°C Duration: grown with Macaca mulatta kidney cells, ATCCCCL 7.1 Protocol: ATCCNO: 30100 SPEC: Grown with Macaca mulatta kidney cells, ATCCCCL 7.1. Kidney cells for use with the amebae are grown in screw-capped tubes containing 2.0 ml of ATCCmedium 722. When tissue cultures are well established, inoculate with 0.1 ml from a flourishing amebae parent culture. Cultures must be gassed with 10% CO2 and 90% air after inoculation and at 4-day intervals. Incubate inoculated amebae cultures on a slant.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Can be cultivated in ATCCmedium 1034 without the cell line.
References: 4585: Cerva L, Novak K. Amoebic meningoencephalitis: 16 fatalities. Science 160: 92, 1968. PubMed: 5642317 5111: Cerva L, et al. An outbreak of acute, fatal amebic meningoencephalitis. Am. J. Epidemiol. 88: 436-444, 1968. PubMed: 5698435
