ATCC? Number:30173?
Organism: Acanthamoeba polyphaga (Puschkarew) Page
Designations: HN-3
Isolation: human nasal swab, Indianapolis, IN, 1965
Depositors: CG Culbertson
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 712: PYGTemperature: 25.0°C Growth Conditions: Axenic Protocol: This strain is distributed as a frozen preparation. See the general procedures for thawing a frozen vial. Once vial is thawed, aseptically transfer the entire contents to a 16 X 125 mm screw-capped test tube or a T-25 tissue culture flask containing 5.0 ml of ATCCmedium 712. Incubate horizontally at 25C.
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Comments: Endosymbiont-bearing. Avirulent. characterization of Acanthamoeba polyphaga [24209] [4673] mitochondrial DNA fingerprinting [21722] phylogeny [24028]
Classification: KINGDOM: Protozoa
References: 4673: Hall J, Voelz H. Bacterial endosymbionts of Acanthamoeba sp.. J. Parasitol. 71: 89-95, 1985. PubMed: 3981353 5114: Culbertson CG, et al. Hartmannella (Acanthamoeba). Experimental chronic, granulomatous brain infections produced by new isolates of low virulence. Am. J. Clin. Pathol. 46: 305-314, 1966. PubMed: 5921408 21722: Gautom RK, et al. Mitochondrial DNA fingerprinting of Acanthamoeba spp. isolated from clinical and environmental sources. J. Clin. Microbiol. 32: 1070-1073, 1994. PubMed: 7913095 24028: Daggett PM, et al. A molecular approach to the phylogeny of Acanthamoeba. Biosystems 18: 399-405, 1985. PubMed: 4084681 24209: Fritsche TR, et al. Occurrence of bacterial endosymbionts in Acanthamoeba spp. isolated from corneal and environmental specimens and contact lenses. J. Clin. Microbiol. 31: 1122-1126, 1993. PubMed: 8501212