ATCC? Number:30153?
Organism: Plasmodium knowlesi Sinton and Mulligan
Designations: Hackeri
Isolation: Anopheles hackeri, West Malaysia, 1961
Depositors: National Institutes of Health
History: ATCC<<--National Institutes of Health<<--R.H. Wharton/D.E. Eyles
Biosafety Level:2
Shipped: frozen
Growth Conditions: Growth Conditions: in vivo, Macaca mulatta Protocol: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. As soon as the strain arrives, remove the frozen ampule from the dry ice and transfer it directly to a 35C water bath. When it is completely thawed, aseptically remove the material with a syringe and inject the entire contents of the vial into an Aotus trivirgatus. This parasite can be maintained by serial passage of parasitized blood (by intravenous inoculation) into monkeys (Aotus trivirgatus).
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Comments: sporozoite transmission [38805] Studies on the transmission [4968] Restrictions: This material is not available for international distribution due to the lack of historical data to support the issuance of a U.S. Fish and Wildlife Service Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES) export permit.
Classification: KINGDOM: Protozoa
References: 4902: Wharton RH, Eyles DE. Anopheles hackeri, a vector of Plasmodium knowlesi in Malaya. Science 134: 279-280, 1961. PubMed: 13784726 4968: Collins WE, et al. Studies on the transmission of simian malaria. IV. Further studies on the transmission of Plasmodium knowlesi by Anopheles balabacensis balabacensis mosquitoes. J. Parasitol. 57: 961-966, 1971. PubMed: 5002525 5131: Coatney GR. The primate malarias. Washington, DC: Government Printing Office; 1971. 38805: Sullivan JS, et al. Sporozoite transmission of three strains of Plasmodium knowlesi to Aotus and Saimiri monkeys. J. Parasitol. 82: 268-271, 1996. PubMed: 8604095