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首頁>美國ATCC>ATCC菌種類>Acanthamoeba comandoni
Acanthamoeba comandoni
    Acanthamoeba comandoni
  • 平臺編號:bio-48682
  • 國際編號:ATCC 30135
  • 拉丁屬名: Acanthamoeba comandoni
  • 規(guī)格:frozen
  • 用途:ATCC原裝進(jìn)口
  • 服務(wù)費(fèi)用:
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  • 注意事項:僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療,非藥用,非食用(產(chǎn)品信息以出庫為準(zhǔn))

ATCC? Number:30135?
Organism: Acanthamoeba comandoni Pussard
Isolation: garden humus, France, ca. 1935
Depositors: W Balamuth
History: ATCC<<--W Balamuth<<--M. Pussard <<--- . . . <<--- J. Comandon/P. de Fonbrune
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 712: PYGTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 30135 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. As soon as the shipment arrives, remove the frozen ampule from the dry ice and transfer it directly to a 35C water bath. After thawing the ampule, transfer the contents to a 16 x 125 mm plastic screw-capped test tube containing 5 ml of fresh medium. (Glass test tubes may also be used, but the cultures can be transferred less frequently when maintained in plastic.) Screw the cap on tightly and incubate the tube on a 5-15 degree slant at the appropriate temperature. Subculture every 2-4 weeks by vigorously agitating the culture and aseptically transferring a 0.1 ml aliquot to a fresh tube of medium. Prolongation of the transfer interval can be extended up to 6 months for certain strains of Acanthamoeba, however, this must be determined empirically for each strain.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Two genetic markers that distinguish pathogenic and nonpathogenic strains [31825] species description [4831] [20723] Restriction fragment length polymorphisms of DNA [5037] phylogeny [24028]
Classification: KINGDOM: Protozoa
References: 4768: Sawyer TK, Griffin JL. Acanthamoeba comandoni and A. astronyxis: taxonomic characteristics of mitotic nuclei, 'centrosomes' and cysts. J. Protozool. 18: 382-388, 1971. 4831: Moura H, et al. Acanthamoeba healyi n. sp. and the isoenzyme and immunoblot profiles of Acanthamoeba spp., groups 1 and 3. J. Protozool. 39: 573-583, 1992. PubMed: 1522539 5037: McLaughlin GL, et al. Restriction fragment length polymorphisms of the DNA of selected Naegleria and Acanthamoeba amebae. J. Clin. Microbiol. 26: 1655-1658, 1988. PubMed: 2903176 20723: Nerad TA, et al. Acanthamoeba pearcei n. sp. (Protozoa: Amoebida) from sewage contaminated sediments. J. Eukaryot. Microbiol. 42: 702-705, 1995. PubMed: 8520585 23896: Daggett PM, et al. Distribution and possible interrelationships of pathogenic and nonpathogenic Acanthamoeba from aquatic environments. Microb. Ecol. 8: 371-386, 1982. 24028: Daggett PM, et al. A molecular approach to the phylogeny of Acanthamoeba. Biosystems 18: 399-405, 1985. PubMed: 4084681 31825: Howe D, et al. Identification of two genetic markers that distinguish pathogenic and nonpathogenic strains of Acanthamoeba spp.. Parasitol. Res. 83: 435-348, 1997. PubMed: 9197389 32162: Stothard DR, et al. The evolutionary history of the genus Acanthamoeba and the identification of eight new 18S rRNA gene sequence types. J. Eukaryot. Microbiol. 45: 45-54, 1998. PubMed: 9495032 52568: Schroeder JM, et al. Use of subgenic 18s ribosomal dna pcr and sequencing for genus and genotype identification of acanthamoebae from humans with keratitis and from sewage sludge. J. Clin. Microbiol. 39: 1903-1911, 2001. PubMed: 11326011 70805: Ledee DR, et al. Advantages of using mitochondrial 16S rDNA sequences to classify clinical isolates of Acanthamoeba. Invest. Ophthalmol. Vis. Sci. 44: 1142-1149, 2003. PubMed: 12601042 71069: Marciano-Cabral F, Cabral G. Acanthamoeba spp. as agents of disease in humans. Clin. Microbiol. Rev. 16: 273-307, 2003. PubMed: 12692099

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