-
平臺編號：bio-48695
-
國際編號：ATCC 30085
-
拉丁屬名： Crithidia fasciculata
-
規(guī)格：frozen
-
用途：ATCC原裝進口
-
服務費用：
加載中…… - 訂購
-
注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
New Orleans-67
ATCC? Number:30085?
Organism: Trypanosoma lewisi (Kent) Laveran and Mesnil
Designations: New Orleans-67
Isolation: rat, Rattus norvegicus, New Orleans, 1967
Depositors: RG Yaeger
Biosafety Level:1
Shipped: frozen
Growth Conditions: Duration: in vivo, laboratory rat Protocol: ATCCNO: 30085 SPEC: Inject the entire thawed contents of the ampule intraperitoneally into an adult rat known to be free of blood parasites. The strain should be monitored, especially after infecting with thawed material. To infect additional rats, draw a drop of tail blood into a 1.0 ml tuberculin syringe containing 0.5 ml of sterile buffered anticoagulant, mix, and inoculate 0.1-0.2 ml of the suspension intraperitoneally/rat. Only a few trypanosomes are needed to infect a non-immune rat. However, do not wait until the blood is swarming with trypanosomes. The time for peak parasitemia may be within 4-5 days if large inocula are given. Peak parasitemia from the thawed material may be slightly sooner or later.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Flow cytometric analysis of immune-globulin and complement component C3 on the surface [24124] Sensitivity to alternate complement pathway-mediated lysis [24205] use of plastic ampoules for freeze preservation [23870]
Cross References: Nucleotide (GenBank) : AJ223566 Trypanosoma lewisi, small subunit ribosomal RNA gene.
Classification: KINGDOM: Protozoa
References: 4950: Gannon JT, Linke HA. Growth studies on xenic cultures of Entamoeba gingivalis using established media. Int. J. Parasitol. 19: 835-838, 1989. PubMed: 2635159 23870: Simione FP Jr., et al. The use of plastic ampoules for freeze preservation of microorganisms. Cryobiology 14: 500-502, 1977. PubMed: 891238 24072: Sturtevant JE, Balber AE. Trypanosoma lewisi: restriction of alternative complement pathway C3/C5 convertase activity. Exp. Parasitol. 63: 260-271, 1987. PubMed: 3556206 24124: Sturtevant JE, Balber AE. Flow cytometric analysis of immunoglobulin and complement component C3 on the surface of Trypanosoma lewisi. J. Protozool. 33: 197-203, 1986. PubMed: 3525820 24205: Sturtevant JE, Balber AE. Externally disposed membrane polypeptides of intact and protease- treated Trypanosoma lewisi correlated with sensitivity to alternate complement pathway-mediated lysis. Infect. Immun. 42: 869-875, 1983. PubMed: 6358042
Organism: Trypanosoma lewisi (Kent) Laveran and Mesnil
Designations: New Orleans-67
Isolation: rat, Rattus norvegicus, New Orleans, 1967
Depositors: RG Yaeger
Biosafety Level:1
Shipped: frozen
Growth Conditions: Duration: in vivo, laboratory rat Protocol: ATCCNO: 30085 SPEC: Inject the entire thawed contents of the ampule intraperitoneally into an adult rat known to be free of blood parasites. The strain should be monitored, especially after infecting with thawed material. To infect additional rats, draw a drop of tail blood into a 1.0 ml tuberculin syringe containing 0.5 ml of sterile buffered anticoagulant, mix, and inoculate 0.1-0.2 ml of the suspension intraperitoneally/rat. Only a few trypanosomes are needed to infect a non-immune rat. However, do not wait until the blood is swarming with trypanosomes. The time for peak parasitemia may be within 4-5 days if large inocula are given. Peak parasitemia from the thawed material may be slightly sooner or later.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Flow cytometric analysis of immune-globulin and complement component C3 on the surface [24124] Sensitivity to alternate complement pathway-mediated lysis [24205] use of plastic ampoules for freeze preservation [23870]
Cross References: Nucleotide (GenBank) : AJ223566 Trypanosoma lewisi, small subunit ribosomal RNA gene.
Classification: KINGDOM: Protozoa
References: 4950: Gannon JT, Linke HA. Growth studies on xenic cultures of Entamoeba gingivalis using established media. Int. J. Parasitol. 19: 835-838, 1989. PubMed: 2635159 23870: Simione FP Jr., et al. The use of plastic ampoules for freeze preservation of microorganisms. Cryobiology 14: 500-502, 1977. PubMed: 891238 24072: Sturtevant JE, Balber AE. Trypanosoma lewisi: restriction of alternative complement pathway C3/C5 convertase activity. Exp. Parasitol. 63: 260-271, 1987. PubMed: 3556206 24124: Sturtevant JE, Balber AE. Flow cytometric analysis of immunoglobulin and complement component C3 on the surface of Trypanosoma lewisi. J. Protozool. 33: 197-203, 1986. PubMed: 3525820 24205: Sturtevant JE, Balber AE. Externally disposed membrane polypeptides of intact and protease- treated Trypanosoma lewisi correlated with sensitivity to alternate complement pathway-mediated lysis. Infect. Immun. 42: 869-875, 1983. PubMed: 6358042
