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Trichomonas vaginalis Donne 拉丁名

(ATCC 30092) 統(tǒng)一編號

Strain Designations 11769 

Application 用途 Sexually Transmitted Disease Research Biosafety Level 2 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. Isolation Human, Colorado Gen Hosp., Denver, CO, 1958 

Product Format 提供形式 frozen 

Storage Conditions 

Frozen Cultures: -70°C for 1 week; liquid N2 vapor for long term storage 

Freeze-dried Cultures: 2-8°C

Live Cultures: See Protocols section for handling information

Type Strain no 

Comments 注釋 Requirement for saturated and unsaturated fatty acids Geographic variation In vitro susceptibility to metronidazole 

Medium 培養(yǎng)基 ATCC Medium 2154: LYI Entamoeba medium 

ATCC Medium 361: Modified TYM basal medium (ATCC medium 358) with pH adjusted to 6.0 and 0.2-0.5 ml of heat-inactivated horse serum added per tube before use

Growth Conditions 生長條件

Temperature 培養(yǎng)溫度: 35°C 

Atmosphere 需氧情況: Anaerobic 

Culture System: Axenic; pH 6.0 

Cryopreservation

1，Harvest and Preservation Harvest cells from a culture that is at or near peak density by centrifugation at 800 x g for 5 min. The cells grown in a medium containing agar are concentrated by centrifugation, a solid pellet does not form. The soft pellet is resuspended to desired cell concentration with agar-free supernatant. 

2，Adjust the concentration of cells to 2 x 106  to  2 x 107/mL in fresh medium.

3， While cells are centrifuging prepare a 10% (v/v) solution of sterile DMSO in fresh medium. 

1）Add 1.0 mL of DMSO to an ice cold 20 x 150 mm screw-capped test tube. 

2）Place the tube on ice and allow the DMSO to solidify (~5 min) and then add 9.0 mL of ice cold medium. 

3）Invert several times to dissolve the DMSO. 

4）Allow to warm to room temperature. 

4，Mix the cell preparation and the DMSO in equal portions. Thus, the final concentration will be 106 to  107 cells/mL and 5% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should no less than 15 min and no longer than 30 min. 

5，Dispense in 0.5 mL aliquots into 1.0 mL to 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation). Place the vials in a controlled rate freezing unit.  From room temperature cool at -1°C/min to -40°C.  If the freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through the heat of fusion.  At -40°C plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.  

6，Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.  (The cooling rate in this apparatus is approximately -1°C/min.)   

7，The frozen preparations should be stored in either the vapor or liquid phase of a nitrogen refrigerator. Frozen preparations stored below -130°C are stabile indefinitely. Those stored at temperatures above -130°C are progressively less stabile as the storage temperature is elevated. Vials should not be stored above -55°C. 

8，To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial just to a level just above the surface of the frozen material. Do not agitate the vial.

9，Immediately after thawing, do not leave in the water bath, aseptically remove the contents of the ampule and inoculate a 16 x 125 mm screw-capped test tube containing either 9 mL of ATCC medium 361 (completed with serum) or 13 mL ATCC Medium 2154 adjusted to pH 6.0. 

10，Incubate the culture at 35°C with the cap screwed on tightly (tube should be vertical for medium 361 or on a 15° horizontal slant for medium 2154). 

Name of Depositor 寄存者 R Samuels

Year of Origin 收藏年份 1958 

References 參考文獻(xiàn) Cornelius DC, et al. Short report: genetic relatedness of Trichomonas vaginalis reference and clinical isolates. Am. J. Trop. Med. Hyg. 83: 1283-1286, 2010. 

Diamond LS. Axenic cultivation of Trichomonas tenax, the oral flagellate of man. I. Establishment of cultures. J. Protozool. 9: 442-444, 1962. 

Krieger JN, et al. Geographic variation among isolates of Trichomonas vaginalis: demonstration of antigenic heterogeneity by using monoclonal antibodies and the indirect immunofluorescence technique. J. Infect. Dis. 152: 979-984, 1985. PubMed: 2413147 

Samuels R, Stouder DJ. Disc testing of drugs against plated Trichomonas vaginalis. J. Protozool. 9: 249-254, 1962. 

Shorb MS, Lund PG. Requirement of Trichomonads for unidentified growth factors, saturated and unsaturated fatty acids. J. Protozool. 6: 122-130, 1959.