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Wellcome CT
    Wellcome CT
  • 平臺編號:bio-48719
  • 國際編號:ATCC 30027
  • 拉丁屬名: Trypanosoma rhodesiense
  • 規(guī)格:frozen
  • 用途:ATCC原裝進口
  • 服務費用:
    加載中……
  • 訂購
  • 注意事項:僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療,非藥用,非食用(產品信息以出庫為準)

ATCC? Number:30027?
Organism: Trypanosoma rhodesiense Stephens and Fantham
Designations: Wellcome CT
Isolation: human, Tinde, Tanganyika, 1934
Depositors: EJ Tobie
History: ATCC<<--EJ Tobie<<--C.A. Hoare <<--- Wellcome Laboratories <<--- J. Corson
Biosafety Level:2
Shipped: frozen
Growth Conditions: Duration: in vivo, laboratory rat Protocol: ATCCNO: 30018 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. Upon arrival remove the frozen ampoule from the dry ice and transfer directly to a 35C water bath. When completely thawed, aseptically remove the material with a syringe and inject 0.2 ml aliquots of the thawed contents of the ampule intraperitoneally into an adult rats known to be free of blood parasites. Draw a drop of blood from the tail of an infected rat. Add 1.0 ml of mammalian saline (0.8% saline buffered to pH 7.0) to the blood and examine a small amount microscopically. If one or more parasites are seen, proceed as follows: collect parasites into a syringe containing a buffered anticoagulant by either cardiac puncture or from the tail vein of the infected animal. Inoculate 0.2 ml of the diluted blood intraperitoneally into an adult rat known to be free of other blood parasites. Repeat the above procedures approximately every 4 days.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Classification: KINGDOM: Protozoa
References: 4669: . . J. Parasitol. 36: 48-54, 1950. 24024: Jaffe JJ, Meymarian E. Trypanocidal activity of a nitrovinylfuran derivative (SQ 18,506) in mice. Exp. Parasitol. 34: 242-250, 1973. PubMed: 4744841

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