ATCC Number:30035
Organism: Leishmania enriettii Muniz and Medina
Isolation: guinea pig, Cavia sp., Brazil, 1948
Depositors: HG du Buy, EJ Tobie
History: ATCC<<--HG du Buy, EJ Tobie<<--. . . <<--- G.H. Medina
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 1012: Diphasic blood agar mediumTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
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Comments: Promastigotes. lipids [23748]
References: 4904: Dubuy HG, et al. Isolation and characterization of DNA from kinetoplasts of Leishmania enriettii. Science 147: 754-756, 1965. PubMed: 14242028 4950: Gannon JT, Linke HA. Growth studies on xenic cultures of Entamoeba gingivalis using established media. Int. J. Parasitol. 19: 835-838, 1989. PubMed: 2635159 21724: Du Buy HG, et al. Comparison of the DNA's obtained from brain nuclei and mitochondria of mice and from the nuclei and kinetoplasts of Leishmania enriettii. Biochim. Biophys. Acta 123: 298-305, 1966. PubMed: 5970343 23748: Beach DH, et al. Lipids of Leishmania promastigotes. J. Parasitol. 65: 201-216, 1979. PubMed: 448607 23983: Ghione M, Dell'Orto P. Human polypeptidic hormone-like substances in microorganisms. Microbiologica 6: 315-326, 1983. PubMed: 6319976 24237: Du Buy HG, Riley FL. Hybridization between the nuclear and kinetoplast DNA's of Leishmania enriettii and between nuclear and mitochondrial DNA's of mouse liver. Proc. Natl. Acad. Sci. USA 57: 790-797, 1967.