ATCC? Number:30028?
Organism: Trypanosoma conorrhini (Donovan) Shortt and Swaminath
Isolation: kissing bug, Triatoma rubrofasciata, Oahu Island, HI, 1947
Depositors: EJ Tobie
History: ATCC<<--EJ Tobie<<--S. Wood <<--- E.J. Tobie
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 1012: Diphasic blood agar mediumTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Variation in kinetoplast minicircle and maxicircle DNA sequences [24219] use of plastic ampoules for freeze preservation [23870]
Classification: KINGDOM: Protozoa
References: 4586: Wood LT. The cultivation of Trypanosoma conorhini. J. Parasitol. 33: 85, 1947. 5079: Wood LT. The occurence of Trypanosoma conorhini Donovan in the reduviid bug, Triatoma rubrofasciata (Degeer) from Oahy, T.H. Proc. Hawaii. Entomol. Soc. 12: 651, 1946. 23870: Simione FP Jr., et al. The use of plastic ampoules for freeze preservation of microorganisms. Cryobiology 14: 500-502, 1977. PubMed: 891238 24219: Gomez-Eichelmann MC, et al. Comparison of several lizard Leishmania species and strains in terms of kinetoplast minicircle and maxicircle DNA sequences, nuclear chromosomes, and membrane lipids [published erratum appears in Mol. Biochem. Parasitol. 28: 301, 1988]. Mol. Biochem. Parasitol. 27: 143-158, 1988. PubMed: 3344003