ATCC® Number:30019™
Organism: Trypanosoma equiperdum Doflein
Designations: NIH kinetoplastic
Depositors: EJ Tobie
History: ATCC<<--EJ Tobie<<--USDA <<--- Pasteur Institut 1903 (?)
Biosafety Level:3
Shipped: frozen
Growth Conditions: Duration: in vivo, laboratory rat Protocol: ATCCNO: 30018 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. Upon arrival remove the frozen ampoule from the dry ice and transfer directly to a 35C water bath. When completely thawed, aseptically remove the material with a syringe and inject 0.2 ml aliquots of the thawed contents of the ampule intraperitoneally into an adult rats known to be free of blood parasites. Draw a drop of blood from the tail of an infected rat. Add 1.0 ml of mammalian saline (0.8% saline buffered to pH 7.0) to the blood and examine a small amount microscopically. If one or more parasites are seen, proceed as follows: collect parasites into a syringe containing a buffered anticoagulant by either cardiac puncture or from the tail vein of the infected animal. Inoculate 0.2 ml of the diluted blood intraperitoneally into an adult rat known to be free of other blood parasites. Repeat the above procedures approximately every 4 days.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Kinetoplast DNA in dyskinetoplastic strains [4907] Abnormal mitochondrial RNA editing [24194]
Cross References: Nucleotide (GenBank) : U03741 Trypanosoma equiperdum kinetoplast maxicircle 12S rRNA gene/COI gene deletion junction.Nucleotide (GenBank) : U03738 Trypanosoma equiperdum kinetoplast maxicircle rearranged ND4-ND5 region.Nucleotide (GenBank) : U03739 Trypanosoma equiperdum kinetoplast maxicircle variable region.Nucleotide (GenBank) : U03740 Trypanosoma equiperdum kinetoplast maxicircle CR4 region.
Classification: KINGDOM: Protozoa
References: 4907: Hajduk SL. Demonstration of kinetoplast DNA in dyskinetoplastic strains of Trypanosoma equiperdum. Science 191: 858-859, 1976. PubMed: 1251198 5001: Tobie EJ. Loss of the kinetoplast in a strain of Trypanosoma equiperdum. Trans. Am. Microsc. Soc. 70: 251-254, 1951. 24194: Shu HH, Stuart K. Mitochondrial transcripts are processed but are not edited normally in Trypanosoma equiperdum (ATCC30019) which has kDNA sequence deletion and duplication. Nucleic Acids Res. 22: 1696-1700, 1994. PubMed: 8202373
