ATCC? Number:30004?
Organism: Ochromonas danica Pringsheim
Designations: Pringsheim
Isolation: freshwater, Denmark, 1954
Depositors: AM Elliott
History: ATCC<<--AM Elliott<<--S.H. Hutner <<--- E.G. Pringsheim
Biosafety Level:1
Shipped: test tube
Growth Conditions: ATCCmedium 177: Fluid thioglycollate mediumTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 11532 SPEC: This strain is shipped as a growing test tube culture. Upon arrival, remove test tube from sealed plastic envelope, remove plastic seal from cap, loosen the cap, one half turn and incubate on a 15 degree horizontal slant at 20-25 C under 150 foot candles of light (40-50 (Einsteins/meter2 /second). Cells tend to clump on sides and bottom of tube. Subculture every 7-10 d. Screw the cap on tightly, invert the tube several times and aseptically transfer a 0.1 ml aliquot to a fresh tube of medium. Incubate as above. For Vitamin B12 assay, use B12 Ochromonas medium.
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Applications: assay of biotin [58631] assay of thiamine vitamin B1 [5162]
Comments: characterization of mitochondrial genome [4822] [4589] comparison of methods of vitamin analysis [23806] Compounds opposing toxicity of 4-nitroquinoline N-oxide [4589] photosynthetic
Classification: KINGDOM: Chromista
References: 4589: Zahalsky AC, et al. Thermostable compounds opposing toxicity of the carcinogen 4-nitroquinoline N-oxide to flagellates and bacteria. J. Protozool. 10: 421-428, 1963. 4822: Coleman AW, et al. Identification of the mitochondrial genome in the chrysophyte alga Ochromonas danica. J. Protozool. 38: 129-135, 1991. 4885: Baker H, et al. Some actions of sulfonylureas charted with protozoan reagents. Nutr. Rep. Int. 32: 727-735, 1985. 5162: Vitamin assay: tested methods. Weinheim: Verlag Chemie; 1965. 20865: Pringsheim EG. Short report on flagellates and algae. I. Alga-like Chrysophyceae in pure culture. Arch. Mikrobiol. 21: 401-410, 1955. PubMed: 13239152 21725: Grusky GE, Aaronson S. Cytochemical changes in aging Ochromonas; evidence for an alkaline phosphatase. J. Protozool. 16: 686-689, 1969. PubMed: 4903097 21726: Hunter SH, et alResemblances between chloroplasts and mitochondria inferred from flagellates inhibited with the carcinogens 4-nitroquioline, N-oxide and ethionineIn: Hunter SH, et alBiochemistry of chloroplasts2New YorkAcademic Press703-720, 1967 23728: Aaronson S, Bensky B. Effect of aging of a cell population on lipids and drug resistance in Ochromonas danica. J. Protozool. 14: 76-78, 1967. 23786: Sokolski WT, et al. Effect of wetting agents on permeability of Ochromonas danica. J. Protozool. 293-296: 1962.. 23806: Voight MN, et al. Comparison of Protozoan and conventional methods of Vitamin analysis. J. Food Sci. 44: 729-734, 1979. 23888: Voigt MN, et al. Vitamin analysis by microbial and protozoan organisms: response to food preservatives and neutralization salts. J. Food Sci. 44: 724, 1979. 24201: Aaronson S. Study of the cellular action of drugs with protozoa--3. Comparison of the effect of SKF 525-A and related compounds on the multiplication of Ochromonas danica. Biochem. Pharmacol. 15: 1241-1244, 1966. PubMed: 5973167 58631: Frank O, et al. A new assay method for biotin in blood, serum, urine and tissues. Anal. Biochem. 3: 31-39, 1962. PubMed: 13864137