ATCC? Number:30017?
Organism: Trypanosoma theileri Laveran
Isolation: blood - animal (dairy cow)
Depositors: EJ Soulsby
History: ATCC<<--EJ Soulsby<<--A. Sollod
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 482: MPHM mediumTemperature: 35.0°C Duration: axenic Protocol: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
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Comments: This strain is useful for bovine trypanosomiasis and lymphocytosis parallel studies.
Classification: KINGDOM: Protozoa
References: 4591: Sollod AE, Soulsby EJ. Cultivation of Trypanosoma theileri at 37 C in partially defined media. J. Protozool. 15: 463-466, 1968. PubMed: 5703078 21710: Hare WC, et alBovine trypanosomiasis and lymphocytosis parallel studiesIn: Hare WC, et alComparative leukemia research 1969No. 36BaselKargerpp. 504-507, 1970 24063: Hare WC, Soulsby EJ. Stimulation of DNA synthesis in bovine leukocyte cultures by Trypanosoma theileri antigen. J. Parasitol. 55: 973-976, 1969. PubMed: 5391314 24071: Splitter EJ, et al. Gel-diffusion and hemagglutination studies with Trypanosoma theileri. Exp. Parasitol. 20: 160-166, 1967. PubMed: 4962447 24109: Splitter EJ, Soulsby EJ. Isolation and continuous cultivation of Trypanosoma theileri in media containing tissue culture fluids. Exp. Parasitol. 21: 137-148, 1967. PubMed: 4169719