ATCC? Number:30021?
Organism: Crypthecodinium cohnii (Seligo) Javornicky
Designations: Puerto Rico
Isolation: seawater, Puerto Rico, 1963
Depositors: K Gold
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 460: A2E6 mediumTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 30021 SPEC: This culture is routinely shipped as a frozen stabilate. Thaw the ampule and aseptically transfer the material to 5 ml of ATCCmedium 460 in a 16 x 125 mm screw-capped test tube. Do not distribute the thawed material to a larger volume of medium. It is essential to first establish the culture in a small volume. Screw cap on tightly, loosen one half turn, and incubate culture upright at 25C. The culture should be ready to subculture in approximately 7 days. To subculture, screw the cap on tightly, invert the culture 5 times and aseptically transfer a 0.1 ml aliquot to 5 ml of ATCCmedium 460 and incubate as above. Prepare two subcultures weekly. Retain all cultures for up to one month to ensure against loss.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: assay of thiamine vitamin B1 [58264] produces docosahexaenoic acids docosahexaenoic acid [5190] [5124]
Comments: Minor Sibling Species G growth requirement [4753] cryopreservation [23828]
Classification: KINGDOM: Protozoa
References: 4753: Gold K, Baren CF. Growth requirements of Gyrodinium cohnii. J. Protozool. 13: 255-257, 1966. 4886: Provasoli L, Gold K. Nutrition of the American strain of Gyrodinium cohnii. Arch. Mikrobiol. 42: 196-203, 1962. PubMed: 14489006 4895: Beam CA, Himes M. Evidence for sexual fusion and recombination in the dinoflagellate Crypthecodinium (Gyrodinium) cohnii. Nature 250: 435-436, 1974. PubMed: 4851938 5124: Seafood products having good taste containing marine microalgae, particularly Crythecodinium cohnii containing docosahexaenoic acid. Japanese Patent 7,008,221 5190: Iizuka T, et al. Marine micro-algae food material containing docosahexaenoic acid, food containing the same and manufacturing method therefor. US Patent 5,547,699 dated Aug 20 1996 23828: Simione FP Jr., Daggett PM. Recovery of a marine dinoflagellate following controlled and uncontrolled freezing. Cryobiology 14: 362-366, 1977. PubMed: 560941 32884: . Sub-culture of a microscopic marine alga. Japanese Patent 7,008,266 32885: . Culture of marine microalgae producing docosahexaenoic acid. Japanese Patent 7,087,959 32888: . Culture of marine microalgae producing docosahexaenoic acid used for e.g. cholesterol lowering action. Japanese Patent 7,087,988 58264: . Handbook of phycological methods. Cambridge: Cambridge Univ. Press; 1975.