ATCC? Number:16523?
Organism: Prototheca wickerhamii Tubaki and Soneda
Designations: PR-28 [UTEX 1437]
Isolation: bovine mastitis, Georgia, (?)
Depositors: WB Cooke
History: ATCC<<--WB Cooke<<--NRRL Y-2464
Biosafety Level:2
Shipped: freeze-dried
Growth Conditions: ATCCmedium 28: Emmons' modification of Sabouraud's agarTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 16522 SPEC: This strain is distributed as a freeze-dried preparation. See the general procedures for opening a freeze-dried vial. Aseptically add 0.5 ml of ice cold medium to containing 12% sucrose to the freeze-dried inner shell vial. Once completely rehydrated, aseptically transfer the material to a 100 mm agar plate of the appropriate medium and evenly distribute the material over the surface of the agar with a spread bar. Subculture 4-6 weeks when incubated at 25C. Subculture 6-12 months when incubated at 18C To subculture, transfer a loopful of material to a fresh plate and spread evenly over the surface.
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Classification: KINGDOM: Plantae
References: 4880: Pore RS. Prototheca taxonomy. Mycopathologia 90: 129-139, 1985.