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ATCC? Number:12858?
Organism: Crithidia fasciculata Leger
Designations: Noller
Isolation: mosquito, Culex sp., Berlin, Germany, 1917
Depositors: HN Guttman
History: ATCC<<--HN Guttman<<--W. Noller
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 355: Crithidia mediumTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
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Comments: Assay of unconjugated pteridines  [5137] Ornithine-arginine metabolism  [4786] Riboprinting and taxonomy  [23607] Proteolytic activities  [23776] isoenzyme electrophoresis for species identification  [24036] Multiple distinct site-specific elements in miniexon arrays  [24177] use of mutants in detecting genetic recombination  [24190] endonuclease-generated fragments of K-DNA, esterase isoenzymes, surface proteins for species identification  [24216] effect of temperature and osmolarity on growth  [30421]
References:  4754: Guttman HN. Patterns of methionine and lysine biosynthesis in the trypanosomatidae during growth.  J. Protozool. 14: 267-271, 1967. PubMed: 6038035 4786: Figueiredo EN, et al. Enzymes of the ornithine-arginine metabolism of trypanosomatids of the genus Crithidia.  J. Protozool. 25: 546-549, 1978. 4857: . .  Exp. Parasitol. 14: 139-142, 1963. 5137:  Analytical microbiology. vol. 2New York: Academic Press; 1972. 5156: Noller W. Blut- und Insektenflagellaten Zuchtung auf Platten.  Arch. Schiffs. Trop-Hyg. 21: 53-94, 1917. 23607: Clark CG. Riboprinting: A tool for the study of genetic diversity in microorganisms.  J. Eukaryot. Microbiol. 44: 277-283, 1997. PubMed: 9225441 23776: Camargo EP, et al. Proteolytic activities in cell extracts of trypanosomatids.  J. Parasitol. 64: 1120-1121, 1978. PubMed: 739304 24036: Goncalves de Lima VM, et al. Five trypanosomatid species of insects distinguished by isoenzymes.  J. Protozool. 26: 648-652, 1979. PubMed: 161788 24177: Teng SC, et al. A new non-LTR retrotransposon provides evidence for multiple distinct site-specific elements in Crithidia fasciculata miniexon arrays.  Nucleic Acids Res. 23: 2929-2936, 1995. PubMed: 7659515 24190: Glassberg J, et al. Isolation and partial characterization of mutants of the trypanosomatid Crithidia fasciculata and their use in detecting genetic recombination.  J. Protozool. 32: 118-125, 1985. PubMed: 3857343 24216: Camargo EP, et al. Electrophoretic analysis of endonuclease-generated fragments of k-DNA, of esterase isoenzymes, and of surface proteins as aids for species identification of insect trypanosomatids.  J. Protozool. 29: 251-258, 1982. PubMed: 6284925 30421: Da Silva JB, Roitman I. Effect of temperature and osmolarity on growth of Crithidia fasciculata, Crithidia hutneri, Crithidia thermophila, and Herpetomonas samuelpessoai.  J. Eukaryot. Microbiol. 29: 269-272, 1982.