ATCC? Number:11532?
Organism: Poterioochromonas malhamensis (Pringsheim) Peterfi
Depositors: SH Hunter
History: ATCC<<--SH Hunter<<--E.G. Pringsheim 1950
Biosafety Level:1
Shipped: test tube
Growth Conditions: ATCCmedium 891: Ochromonas mediumTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 11532 SPEC: This strain is shipped as a growing test tube culture. Upon arrival, remove test tube from sealed plastic envelope, remove plastic seal from cap, loosen the cap, one half turn and incubate on a 15 degree horizontal slant at 20-25 C under 150 foot candles of light (40-50 (Einsteins/meter2 /second). Cells tend to clump on sides and bottom of tube. Subculture every 7-10 d. Screw the cap on tightly, invert the tube several times and aseptically transfer a 0.1 ml aliquot to a fresh tube of medium. Incubate as above. For Vitamin B12 assay, use B12 Ochromonas medium.
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Applications: assay of vitamin B12 cobalamin, cobalamins [5138] [23643] [45899]
Comments: characterization of mitochondrial genome [4822] comparison of methods of vitamin analysis [23806] water-soluble vitamins in cells and spent culture supernatants [23950] photosynthetic
Classification: KINGDOM: Chromista
References: 4822: Coleman AW, et al. Identification of the mitochondrial genome in the chrysophyte alga Ochromonas danica. J. Protozool. 38: 129-135, 1991. 5138: Analytical microbiology. New York: Academic Press; 1963. 23643: . Analytical microbiology. New York: Academic Press; 1963. 23806: Voight MN, et al. Comparison of Protozoan and conventional methods of Vitamin analysis. J. Food Sci. 44: 729-734, 1979. 23888: Voigt MN, et al. Vitamin analysis by microbial and protozoan organisms: response to food preservatives and neutralization salts. J. Food Sci. 44: 724, 1979. 23950: Baker ER, et al. Water-soluble vitamins in cells and spent culture supernatants of Poteriochronous stipitata, Euglena gracilis and Tetrahymena thermophila. Arch. Microbiol. 129: 310-313, 1981. 23983: Ghione M, Dell'Orto P. Human polypeptidic hormone-like substances in microorganisms. Microbiologica 6: 315-326, 1983. PubMed: 6319976 45899: Ford JE. The microbiological assay of 'vitamin B12'; the specificity of the requirement of Ochromonas malhamensis for cyanocobalamin. Br. J. Nutr. 7: 299-306, 1953.