ATCC? Number:97541?
Host Organism: Salmonella enterica subsp. enterica (ex Kauffmann and Edwards) Le Minor and Popoff serovar Typhimurium ATCC19585
Designations: r34
Depositors: National Institutes of Health/NIMH, CR Merril, National Institutes of Health/NIMH
Biosafety Level:1
Shipped: freeze-dried bacteria-free lysate
Growth Conditions: Temperature: 37.0°C
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: potential bacteremia therapeutic agent [5201] This is one of six bacteriophage strains deposited at the ATCC(97537-97539 are lambda strains and 97540-97542 are P22 strains) that remain in the mouse circulatory system for long periods of time. They are potential therapeutic agents for bacteremias.
Comments: Recommendation for recovery of bacteriophage is to adsorb lysate to overnight grown S. typhimurium LT2 at 37C for 18 minutes, plate adsorbtion mixture onto LB agar plates, and then incubate overnight at 37C.
References : 5201: Merril CR, et al. Long-circulating bacteriophage as antibacterial agents. Proc. Natl. Acad. Sci. USA 93: 3188-3192, 1996. PubMed: 8622911