ATCC? Number:VR-301?
Classification: Picornaviridae, Enterovirus, Poliovirus
Agent: Human poliovirus 2 (attenuated) deposited as Poliovirus 2 (attenuated)
Strain: W-2
Original Source: Derived by passage of the TN strain (originally from an asymptomatic case of poliomyelitis)
Depositors: H Koprowski
Biosafety Level:2
Shipped: frozen
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Host Organism : monkey kidney cells
Incubation : Duration: 3-7 days
Effect : Yes, in vitro effects: Cytopathic effects in tissue culture
Comments : [The BMBL notes that all laboratory personnel working directly with the agent must have documented polio vaccination or demonstrated serologic evidence of immunity to all three poliovirus types. (CDC/NIH Biosafety in Microbiological and Biomedical Laboratories, 4th ed, 1999 - stock no. 017-040-00547-4)]. This attenuated W-II strain of polio type 2 was developed as a human vaccine candidate by passage of the mouse adapted TN strain at low temperatures (23-30C).
References : 33474: Carp RI, et al. Modification of an attenuated type 2 polio vaccine following passage at 23degreeC. Proc. Soc. Exp. Biol. Med. 112: 251-256, 1963. PubMed: 14018807