-
平臺編號：bio-49394
-
國際編號：ATCC VR-1354
-
拉丁屬名： Vaccinia virus
-
規(guī)格：frozen
-
用途：ATCC原裝進口
-
服務(wù)費用：
加載中…… - 訂購
-
注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
中文名稱暫無
ATCC? Number:VR-1354?
Classification: Poxviridae, Orthopoxvirus
Agent: Vaccinia virus deposited as Vaccinia virus, Orthopoxvirus
Strain: WR (NIH TC-adapted)
Original Source: multiple laboratory passages in cell culture of WR strain of vaccinia
Depositors: NS Cooper, B Moss
Biosafety Level:2
Shipped: frozen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Incubation : Temperature: 37.0°C Duration: 2-3 days at 37C
Effect : Yes, in vitro effects: Cytopathic effects (localized enlarged, rounded cells which eventually extend throughout the monolayer)
Comments : This strain is recommended by ATCCfor use in the tests described in Fed. Spec. A-A-1438 where only the taxon is specified. The host range includes many mammalian and avian cells, e.g.,HeLa S3 (ATCCCCL-2.2) and BS-C-1 (ATCCCCL-26). This tissue culture adapted WR strain of vaccinia is derived from ATCCVR-119 and has been shown to serve as an effective expression vector for foreign genes (Mackett et al., J. Virol., 1984) Dr. Moss received the WR strain in 1966 from Dr. Salzman who obtained the virus as VR-119 from the ATCC. The tissue culture adapted strain WR has been widely distributed and used in constructing vectors for gene expression (J. Gen Virol, 1986). Vaccination recommended for lab staff manipulating vaccinia, monkeypox, or cowpox viruses (CDC/NIH Biosafety in Microbiological and Biomedical Laboratories, 4th ed, 1999 - stock no. 017-040-00547-4). Cell associated virus from infected HeLa cells
Related Products: recommended serum:ATCC 30-2020
References : 32840: Salzman NP, Sebring ED. Sequential formation of vaccinia virus proteins and viral deoxyribonucleic acid replication. J. Virol. 1: 16-23, 1967. PubMed: 4248263 32841: Mackett M, et al. Vaccinia virus: a selectable eukaryotic cloning and expression vector. Proc. Natl. Acad. Sci. USA 79: 7415-7419, 1982. PubMed: 6296831
