ATCC? Number:VR-2551?
Classification: Reoviridae, Rotavirus
Agent: Human rotavirus
Strain: WI61
Original Source: human infant, Philadelphia, PA
Depositors: Children's Hospital of Philadelphia, Wistar Institute of Anatomy and Biology
Biosafety Level:2
Shipped: frozen
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Host Organism : MA-104 cells (ATCCCRL-2378) MA-104 cells (ATCCCRL-2378); CV-1 cells (ATCCCCL-70)
Incubation : Atmosphere: 5% CO2 in air recommended Temperature: 36.0°C Duration: 1-4 days at 36C
Effect : Yes, in vitro effects: Cytopathic effects (rounding and detachment of cells, plaque formation) in MA-104 cells with massive cell destruction in 5 to 6 days
Store at : -70 C or colder
Comments : may cause gastroenteritis in infants -20C to -70C Propagate in cells fed with serum-free medium containing 0.5 ug/ml purified trypsin.