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首頁>美國ATCC>ATCC病毒類> Vaccinia virus deposited as Vaccinia virus,
 Vaccinia virus deposited as Vaccinia virus,
    Vaccinia virus deposited as Vaccinia virus,
  • 平臺(tái)編號(hào):bio-49420
  • 國際編號(hào):ATCC VR-2056
  • 拉丁屬名: Vaccinia virus deposited as Vaccinia virus
  • 規(guī)格:frozen
  • 用途:ATCC原裝進(jìn)口
  • 服務(wù)費(fèi)用:
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  • 訂購
  • 注意事項(xiàng):僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療,非藥用,非食用(產(chǎn)品信息以出庫為準(zhǔn))

ATCC? Number:VR-2056?
Classification: Poxviridae, Orthopoxvirus
Agent: Vaccinia virus deposited as Vaccinia virus, Orthopoxvirus
Strain: VTK-79L
Depositors: New York State Department of Health
Biosafety Level:2
Shipped: frozen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Host Organism : HeLa cells (ATCCCCL-2); CV-1 cells (ATCCCCL-70); Vero cells (ATCCCCL-81); chicken embryo fibroblasts
Effect : Yes, in vitro effects: Cytopathic effects (plaque formation) in Vero cells
Comments : Vaccination recommended for lab staff manipulating vaccinia, monkeypox, or cowpox viruses (CDC/NIH Biosafety in Microbiological and Biomedical Laboratories, 4th ed, 1999 - stock no. 017-040-00547-4). A thymidine kinase negative variant of the S-variant of vaccinia strain WR. This is a thymidine kinase negative (TK-) mutant of the L-variant of vaccinia virus strain WR. Plasmids can be introduced into this mutant by in vivo recombination. [13123] The HindIII J-fragment of TK-79 containing a mutant (non-functional) thymidine kinase (TK) gene was cloned into the L-variant vaccinia virus by in vivo recombination. [13123] Prepared by: E. Paoletti and D. Panicali
References : 13123: Paoletti E, Panicali D. Modified vaccinia virus. US Patent 4,603,112 dated Jul 29 1986 13130: Paoletti E, Panicali D. Method for immunizing animals with synthetically modified vaccinia virus. US Patent 4,722,848 dated Feb 2 1988 13139: Panicali D, et al. Two major DNA variants present in serially propagated stocks of the WR strain of vaccinia virus. J. Virol. 37: 1000-1010, 1981. PubMed: 6262520

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