ATCC? Number:VR-2061?
Classification: Poxviridae, Orthopoxvirus
Agent: Vaccinia virus
Strain: vP-59 [strain VP59]
Original Source: New York State Department of Health strain
Depositors: E Paoletti
Biosafety Level:2
Shipped: frozen
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Host Organism : HeLa cells (ATCCCCL-2); BHK-21 cells (ATCCCCL-10); chicken embryo fibroblasts
Effect : Yes, in vitro effects: Cytopathic effects in tissue culture
Comments : The level of expression from this clone is several thousand times greater than that of vP-11 (ATCCVR-2045). [13130] A recombinant of vaccinia (VTK-79) containing the HBV gene from ATCC39735 and expresses HBV surface antigen. This clone expresses HBsAg and can be used in the production of antibodies against this protein. [13130] A HindIII fragment encoding HBsAg was cloned into VTK-79 (ATCCVR-2031) by in vivo recombination. [13130]
References : 13123: Paoletti E, Panicali D. Modified vaccinia virus. US Patent 4,603,112 dated Jul 29 1986 13130: Paoletti E, Panicali D. Method for immunizing animals with synthetically modified vaccinia virus. US Patent 4,722,848 dated Feb 2 1988 13139: Panicali D, et al. Two major DNA variants present in serially propagated stocks of the WR strain of vaccinia virus. J. Virol. 37: 1000-1010, 1981. PubMed: 6262520 33253: Biberfield. . J. Immunol. Methods 6: 249-259, 1974. 33254: Moss. . Chem. Abstr. 95: 217, 1981.