ATCC? Number:VR-2031?
Classification: Poxviridae, Orthopoxvirus
Agent: Vaccinia virus deposited as Vaccinia virus, Orthopoxvirus
Strain: Vtk-79
Depositors: New York State Department of Health
Biosafety Level:2
Shipped: frozen
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Host Organism : HeLa cells (ATCCCCL-75); CV-1 cells (ATCCCCL-70)
Incubation : Duration: 5 days
Effect : Yes, in vitro effects: Cytopathic effects in tissue culture
Comments : Vaccination recommended for lab staff manipulating vaccinia, monkeypox, or cowpox viruses (CDC/NIH Biosafety in Microbiological and Biomedical Laboratories, 4th ed, 1999 - stock no. 017-040-00547-4). A thymidine kinase negative variant of the S-variant of vaccinia strain WR. A thymidine kinase negative (TK-) mutant of the S-variant of vaccinia virus strain WR. Plasmids can be introduced into this mutant by in vivo recombination. [13130] Prepared by: E. Paoletti Isolated from an S-variant vaccinia population subjected to strong selective pressure by growing in cells in the presence of 5-bromo-2'-deoxyuridine. [13130]
References : 13123: Paoletti E, Panicali D. Modified vaccinia virus. US Patent 4,603,112 dated Jul 29 1986 13130: Paoletti E, Panicali D. Method for immunizing animals with synthetically modified vaccinia virus. US Patent 4,722,848 dated Feb 2 1988 13139: Panicali D, et al. Two major DNA variants present in serially propagated stocks of the WR strain of vaccinia virus. J. Virol. 37: 1000-1010, 1981. PubMed: 6262520