>美國(guó)ATCC>ATCC菌種類>G型沙眼衣原體
Chlamydia trachomatis Serovar G 拉丁名
(ATCC? VR-878?) 統(tǒng)一編號(hào)
Classification Chlamydiaceae
Deposited As Trachoma serotype g Agent Chlamydia trachomatis Serovar G Strain UW-57/Cx
Applications 用途 Sexually Transmitted Disease Research
Biosafety Level 生物安全等級(jí) 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 frozen 1 mL per vial
Storage Conditions 保藏方法 -70°C or colder
Comments 注釋 Note that activities with high potential for aerosol production require BSL 3 facilities and practices.
Add glass beads and vortex preparation to disrupt cells. Infect monolayer with disrupted material.
Centrifuge at 3000 x rpm (750 x g) for one hour. Feed with fresh growth medium containing FBS prescreened for Chlamydia antibodies and 1-2 μg/mL cycloheximide.
Incubate at 36°C for 72 hours.
Effect on Host Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific monoclonal antibodies or Giemsa
Recommended Host McCoy cells (ATCC? CRL-1696?)
Growth Conditions 生長(zhǎng)條件
Temperature 培養(yǎng)溫度 : 36°C
Recommendations For Infection: For best results cells should be 24 to 48 hours old and 80-90% confluent (not 100% confluent). Incubation: 3 days, a 5% CO2 in air atmosphere is recommended
Effect on Host Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific monoclonal antibodies or Giemsa
Name of Depositor 寄存人 SP Wang Source Human cervix (epithelial tissue), cervicitis, Seattle, WA, 1971
References 參考文獻(xiàn) Wang SP, et al. Three new immunologic types of trachoma-inclusion conjunctivitis organisms. J. Immunol. 110: 873-879, 1973. PubMed: 4631875