ATCC? Number:VR-2048?
Classification: Poxviridae, Orthopoxvirus
Agent: Vaccinia virus
Strain: vP-14
Depositors: E Paoletti
Biosafety Level:2
Shipped: frozen
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Host Organism : HeLa cells (ATCCCCL-2); CV-1 (ATCCCCL-70); Vero (ATCCCCL-81); chicken embryo fibroblast
Effect : Yes, in vitro effects: Cytopathic effects in tissue culture
Comments : vP-14 (ATCCVR-2048) and vP-16 (ATCCVR-2050) contain the same insert but in opposite orientations. [13130] Recombinant virus consisting of an Eco R1 HSV F-fragment insert in vaccinia Vp7. An EcoRI fragment coding for the herpes virus type I glycoproteins gA + gB was cloned into vP-7 (ATCCVR-2042) by in vivo recombination. [13130]
References : 13123: Paoletti E, Panicali D. Modified vaccinia virus. US Patent 4,603,112 dated Jul 29 1986 13130: Paoletti E, Panicali D. Method for immunizing animals with synthetically modified vaccinia virus. US Patent 4,722,848 dated Feb 2 1988 13139: Panicali D, et al. Two major DNA variants present in serially propagated stocks of the WR strain of vaccinia virus. J. Virol. 37: 1000-1010, 1981. PubMed: 6262520