-
平臺(tái)編號(hào)：bio-49464
-
國(guó)際編號(hào)：ATCC VR-1356
-
拉丁屬名： Chlamydophila pneumoniae
-
規(guī)格：frozen
-
用途：ATCC原裝進(jìn)口
-
服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
-
注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
中文名稱暫無(wú)
ATCC? Number:VR-1356?
Classification: Chlamydiacea, Chlamydophila
Agent: Chlamydophila pneumoniae deposited as Chlamydia pneumoniae Grayston et al.
Strain: TWAR strain 2023
Original Source: nasopharynx of human patient with pneumonia
Depositors: MR Hammerschlag
Biosafety Level:2
Shipped: frozen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Host Organism : HEp-2 cells (ATCCCCL-23) Human; human cell lines; HeLa 229 cells (ATCCCCL-2.1)
Incubation : Duration: 2-3 days at 37.0°C in HEp-2 cells (ATCCCCL-23)
Effect : Yes, in vitro effects: intracellular inclusion bodies visualized by fluorescent staining with genus or species specific conjugated antibodies
Store at : -70.0°C or colder
Comments : This preparation has tested positive for Mycoplasma contamination. Note that activities with high potential for aerosol production require BSL 3 facilities and practices. Forms intracellular inclusions which react with specific anti-C. pneumoniae monoclonal antibodies and monoclonal antibodies directed against the chlamydia LPS. Inclusions will not stain with iodine or monoclonal antibodies against the MOMP of C. trachomatis. Infection is enhanced by centrifugation and use of cycloheximide (1 to 2 mcg/ml) in medium. Suggested protocol for propagation: Add glass beads and vortex preparation to disrupt cells. Infect monolayer with disrupted material. Centrifuge at 3000 x rpm (750 x g) for 1 hour. Feed with fresh growth medium containing FBS prescreened for Chlamydia antibodies and 1-2 ug/mL cycloheximide. Incubate at 37.0°C for 72 hours. -70.0°C or colder
Classification: Chlamydiacea, Chlamydophila
Agent: Chlamydophila pneumoniae deposited as Chlamydia pneumoniae Grayston et al.
Strain: TWAR strain 2023
Original Source: nasopharynx of human patient with pneumonia
Depositors: MR Hammerschlag
Biosafety Level:2
Shipped: frozen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Host Organism : HEp-2 cells (ATCCCCL-23) Human; human cell lines; HeLa 229 cells (ATCCCCL-2.1)
Incubation : Duration: 2-3 days at 37.0°C in HEp-2 cells (ATCCCCL-23)
Effect : Yes, in vitro effects: intracellular inclusion bodies visualized by fluorescent staining with genus or species specific conjugated antibodies
Store at : -70.0°C or colder
Comments : This preparation has tested positive for Mycoplasma contamination. Note that activities with high potential for aerosol production require BSL 3 facilities and practices. Forms intracellular inclusions which react with specific anti-C. pneumoniae monoclonal antibodies and monoclonal antibodies directed against the chlamydia LPS. Inclusions will not stain with iodine or monoclonal antibodies against the MOMP of C. trachomatis. Infection is enhanced by centrifugation and use of cycloheximide (1 to 2 mcg/ml) in medium. Suggested protocol for propagation: Add glass beads and vortex preparation to disrupt cells. Infect monolayer with disrupted material. Centrifuge at 3000 x rpm (750 x g) for 1 hour. Feed with fresh growth medium containing FBS prescreened for Chlamydia antibodies and 1-2 ug/mL cycloheximide. Incubate at 37.0°C for 72 hours. -70.0°C or colder
