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Chlamydia trachomatis Serovar I 拉丁名

(ATCC? VR-880?) 統(tǒng)一編號 

Classification Chlamydiacae Deposited As Trachoma serotype i Agent Chlamydia trachomatis Serovar I Strain UW-12/Ur 

Biosafety Level 生物安全等級 2 

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. 

Product Format 提供形式 frozen 1 mL per vial 

Storage Conditions 保藏方法 -70°C or colder 

Comments 注釋 Note that activities with high potential for aerosol production require BSL 3 facilities and practices. 

Add glassbeads and vortex preparation to disrupt cells.  

Infect monolayer with disrupted material.  

Centrifuge at 3000 x rpm (750 x g) for 1 hour.  

Feed with fresh growth medium containing 1-2 μg/mL cycloheximide.  

Incubate at 37°C for 48 hours.

Effect on Host Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific monoclonal antibodies or Giemsa. 

Recommended Host McCoy (ATCC? CRL-1696?)

Growth Conditions 生長條件 

Temperature 培養(yǎng)溫度 : 36°C 

Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 90-100% confluent. 

Prior to infection, disrupt cells by sonication or vortex with sterile glass beads. Remove medium and inoculate with a small volume of inoculum (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 1). 

Adsorb by centrifugation at 1500 x g for 1 hour at 25°C. End adsorption by adding growth medium. Incubation: 1-3 days 

Effect on Host Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific monoclonal antibodies or Giemsa. 

Name of Depositor 寄存人 SP Wang Source Male urethra with non-gonococcal urethritis 

References 參考文獻 Wang SP, et al. Three new immunologic types of trachoma-inclusion conjunctivitis organisms. J. Immunol. 110: 873-879, 1973. PubMed: 4631875