Chlamydia trachomatis Serovar D 拉丁名
(ATCC® VR-885™) 統(tǒng)一編號
Classification Chlamydiaceae, Chlamydia
Deposited As Trachoma serotype d
Agent Chlamydia trachomatis Serovar D
Strain Trachoma type D strain UW-3/Cx
Applications 用途 Sexually Transmitted Disease Research
Biosafety Level 生物安全等級 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 frozen 1 mL per vial
Storage Conditions 保藏條件 -70℃ or colder
Comments 注釋 FBS used to culture Chlamydia must be prescreened to verify that the serum does not contain antibodies to Chlamydia or other factors that would interfere with growth. Note that activities with high potential for aerosol production require BSL 3 facilities and practices. Rapid loss in titer when stored above -70℃. Suggested protocol for propagation: Add glass beads and vortex preparation to disrupt cells. Infect monolayer with disrupted material. Centrifuge at 3000 x rpm (750 x g) for 1 hour. Feed with fresh growth medium containing FBS prescreened for Chlamydia antibodies and 1-2 µg/mL Cycloheximide.
Effect on Host CPE, intracellular inclusion bodies Recommended Host McCoy cells (ATCC® CRL-1696™)
Growth Conditions 生長條件
Temperature 培養(yǎng)溫度: 36℃
Recommendations For Infection: For best results cells should be 24 to 48 hours old and 90-100% confluent.
Incubation: 3 days, 5% CO2 in air atmosphere is recommended
Effect on Host CPE, intracellular inclusion bodies
