ATCC® Number:VR-879™
Classification: Chlamydiaceae
Agent: Chlamydia trachomatis (Busacca) Rake deposited as Trachoma serotype h
Strain: Trachoma type H strain UW-43/Cx
Original Source: human cervix (epithelial tissue), cervicitis, Seattle, WA, 1971
Depositors: SP Wang
Biosafety Level 生物安全等級 :2
Shipped: frozen
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Host Organism : McCoy cells (ATCCCRL-1696) primates; HeLa cells (ATCCCCL-2); HEp-2 cells (ATCCCCL-23) human; non-human primates
Incubation : Duration: 2 days at 37.0℃
Effect : Yes, in vitro effects: Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific monoclonal antibodies or Giemsa
Store at : -70.0℃ or colder
Comments 注釋 : Add glassbeads and vortex preparation to disrupt cells. Infect monolayer with disrupted material. Centrifuge at 3000 x rpm (750 x g) for 1 hour. Feed with fresh growth medium containing 1-2 ug/mL cycloheximide. Incubate at 37C for 48 hours. Note that activities with high potential for aerosol production require BSL 3 facilities and practices.
