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Chlamydia trachomatis 拉丁名

(ATCC? VR-887?) 統(tǒng)一編號

Classification Chlamydiaceae, Chlamydia 

Deposited As trachoma serotype k 

Agent Chlamydia trachomatis 

Strain Trachoma type K strain UW-31/Cx 

Applications 用途 Note that activities with high potential for aerosol production require BSL 3 facilities and practices. 

Biosafety Level 生物安全等級 2 

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. 

Product Format 提供形式 frozen 

Storage Conditions 保藏方法 -70°C or colder 

Comments 注釋 Closely related immunologically with LGV Type L III. 

Note that activities with high potential for aerosol production require BSL 3 facilities and practices. 

Suggested protocol for propagation: Add glass beads and vortex preparation to disrupt cells. 

Infect monolayer with disrupted material. Centrifuge at 3000 x rpm (750 x g) for 1 hour. 

Feed with fresh growth medium containing FBS prescreened for Chlamydia antibodies and 1-2 ug/mL cycloheximide. 

Incubate at 37°C for 48 hours. 

Effect on Host Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific monoclonal antibodies or Giemsa 

Recommended Host McCoy cells (ATCC? CRL-1696?) 

HeLa 229 cells (ATCC? CCL-2.1?) 

Growth Conditions 生長條件 

Temperature 培養(yǎng)溫度 : 36.5°C 

Incubation: 2 days at 37°C 

Effect on Host Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific monoclonal antibodies or Giemsa 

Name of Depositor 寄存人 SP Wang Source Human cervix, cervicitis, Seattle, WA 

References 參考文獻(xiàn) Kuo CC, et al. TRIC type K, a new immunologic type of Chlamydia trachomatis. J. Immunol. 113: 591-596, 1974. PubMed: 4210884

and 1-2 ug/mL cycloheximide. Incubate at 37C for 48 hours.