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ATCC? Number:VR-1739?
Classification: Reoviridae, Rotavirus
Agent: Rhesus rotavirus (MMU 18006)
Strain: S:USA:79:2
Original Source: Stool sample from Macaca mulatta monkey with diarrhea. This product has been transferred into a different host at ATCC.
Depositors: RW Emmons and NJ Schmidt
Biosafety Level:2
Shipped: frozen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Host Organism : Production Host: MA104.1 (ATCCCRL-2378.1) Alternate Hosts: BS-C-1 (ATCCCCL-26), Rhesus embryonic monkey kidney cells, Primary cynomolgus monkey kidney cells
Incubation : For best results, plate cells 24-48 hours in advance. Infect at 80%-90% confluent, not 100% confluent. Remove medium and inoculate with a small volume of virus diluted to provide a MOI of about 0.1. Adsorb 1 hour at 37.0°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubate at 37.0°C in a humidified, 5% CO2 atmosphere for 2-3 days, until CPE are well advanced in 100% of the culture. In TC, ATCCVR-1739 should be grown in serum free media that contains 2.0μg/ml trypsin.
Effect : CPE in the form of refractile, rounded cells throughout monolayer or in loose clusters.
Store at : -70.0°C or colder
Comments : Derived by adaptation of primary cell culture product ATCCVR-954 to MA104.1 (ATCCCRL-2378.1) cells. (Note: ATCCVR-954 and ATCCVR-1739 have not been compared with respect to sequence or infectivity in primary cell culture and tissue culture).