ATCC? Number:VR-2379?
Classification: Poxviridae, Orthopoxvirus
Agent: Vaccinia virus
Strain: RPmuhr23HA+ [recombinant of Utrecht strain RPuhr23]
Original Source: Vaccinia/rabbitpox recombinant
Depositors: University of Florida, Gainesville, RW Moyer, University of Florida, Gainesville
Biosafety Level:2
Shipped: frozen
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Host Organism : CV-1 (ATCCCCL 70); chicken embryo fibroblasts
Effect : Yes, in vitro effects: Cytopathic effects in tissue culture
Comments : Sequences cloned into pHGN3 (ATCC69051) can be integrated into this virus by transfection of a virus-infected host. [40815] Recombinants can be identified by inactivation of the hemagglutinin gene (HA-). [40815] This is a potential human pathogen. Parent strain RPuhr23 is a spontaneous white pock mutant of wild-type rabbitpox. The HA inserted in ATCCVR-2379 can serve as a cloning site for genes affecting vaccinia and rabbitpox. ATCC69051 is a shuttle vector for use with this system. Avirulent, hemagglutinin positive (HA+) recombinant, derived from the white pock deletion mutant of rabbit poxvirus. Part of a virulence testing system to be used with pHGN3 (ATCC69051). [40815] The insert contains approximately 443 bp 5' flanking sequence, 988 bp coding sequence and 408 bp 3' flanking sequence. [40815] Constructed by introducing the hemagglutinin gene from vaccinia virus IHD-J by homologous recombination. [40815]
References : 40815: Moyer RW, Bloom DC. Biological system for constructing and testing viral vaccines. US Patent 5,212,057 dated May 18 1993