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平臺編號：bio-49755
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國際編號：ATCC VR-2212
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拉丁屬名： Raccoonpox virus
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規(guī)格：frozen
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用途：ATCC原裝進(jìn)口
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
Raccoonpox virus
ATCC? Number:VR-2212?
Classification: Poxviridae, Orthopoxvirus
Agent: Raccoonpox virus deposited as Raccoonpox virus, Orthopoxvirus
Strain: RCN KB3-JE-13 (rabG) [CVS Rabiesvirus Glycoprotein recombinant]
Original Source: Recombinant Rabies vaccine
Depositors: JJ Esposito
Biosafety Level:2
Shipped: frozen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Host Organism : CV-1 cells (ATCCCCL-70); Rat2 cells (ATCCCRL-1764; BSC40 cells primate cell lines; Vero cells (ATCCCCL-81); HeLa cells (ATCCCCL-2); chicken chorioallantoic membrane; rat 2 (TK-) cells
Incubation : Temperature: 36.0°C Duration: incubate at 36C
Effect : Yes, in vitro effects: Cytopathic effects (cell rounding, syncytium formation)
Comments : Raccoon poxvirus CDC # V71-I-85A was plaque purified three times then plasmid pKB3-JE-13 (ATCC40280) was transfected to cause insertion of CVS rabies glycoprotein cDNA in the thymidine kinase gene, inactivating it. Plaque titration is on CV-1, BSC40 or Rat2 cells. Adsorb virus for 2 hours at 36C, overlay with DMEM + 2% FBS (liquid overlay is OK) incubate for 72 hours, stain monolayer with 0.01% crystal violet in 10% formalin, rinse with PBS.
References : 33385: Esposito JJ, Baer GM. Raccoon poxvirus as a gene expression and vaccine vector for genes of rabies virus and other organisms. US Patent 5,266,313 dated Nov 30 1993 33387: Esposito JJ, et al. Successful oral rabies vaccination of raccoons with raccoon poxvirus recombinants expressing rabies virus glycoprotein. Virology 165: 313-316, 1988. PubMed: 3291388 33388: EspositoModern approaches to new vaccines including prevention of AIDS.In: EspositoVaccines 92321-330, 1992
Classification: Poxviridae, Orthopoxvirus
Agent: Raccoonpox virus deposited as Raccoonpox virus, Orthopoxvirus
Strain: RCN KB3-JE-13 (rabG) [CVS Rabiesvirus Glycoprotein recombinant]
Original Source: Recombinant Rabies vaccine
Depositors: JJ Esposito
Biosafety Level:2
Shipped: frozen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Host Organism : CV-1 cells (ATCCCCL-70); Rat2 cells (ATCCCRL-1764; BSC40 cells primate cell lines; Vero cells (ATCCCCL-81); HeLa cells (ATCCCCL-2); chicken chorioallantoic membrane; rat 2 (TK-) cells
Incubation : Temperature: 36.0°C Duration: incubate at 36C
Effect : Yes, in vitro effects: Cytopathic effects (cell rounding, syncytium formation)
Comments : Raccoon poxvirus CDC # V71-I-85A was plaque purified three times then plasmid pKB3-JE-13 (ATCC40280) was transfected to cause insertion of CVS rabies glycoprotein cDNA in the thymidine kinase gene, inactivating it. Plaque titration is on CV-1, BSC40 or Rat2 cells. Adsorb virus for 2 hours at 36C, overlay with DMEM + 2% FBS (liquid overlay is OK) incubate for 72 hours, stain monolayer with 0.01% crystal violet in 10% formalin, rinse with PBS.
References : 33385: Esposito JJ, Baer GM. Raccoon poxvirus as a gene expression and vaccine vector for genes of rabies virus and other organisms. US Patent 5,266,313 dated Nov 30 1993 33387: Esposito JJ, et al. Successful oral rabies vaccination of raccoons with raccoon poxvirus recombinants expressing rabies virus glycoprotein. Virology 165: 313-316, 1988. PubMed: 3291388 33388: EspositoModern approaches to new vaccines including prevention of AIDS.In: EspositoVaccines 92321-330, 1992
