ATCC? Number:VR-186?
Classification: Picornaviridae, Enterovirus, Human enterovirus B
Agent: Human Coxsackievirus A 9 deposited as Coxsackievirus A9
Strain: P.B. (Bozek)
Original Source: Stool from 6-year-old child, New York, 1950
Depositors: HA Wenner
Biosafety Level:2
Shipped: frozen
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Host Organism : monkey kidney cells; suckling mouse, i.c. or i.p. inoculation monkey kidney; human diploid embryonic, human amnion cells for in tissue culture material; suckling mouse for mouse passage material
Incubation : Duration: 2-4 days
Effect : Yes, in vitro effects: Cytopathic effects in tissue culture Yes, in vivo effects: death in suckling mice
Comments : Following reconstitution, dilute lyophilized material at least 1:10 in physiological culture medium prior to infecting cells in vitro. No CPE produced in HeLa cells.
References : 32510: Dalldorf G. The coxsackie virus group. Ann. N.Y. Acad. Sci. 56: 583-586, 1953. PubMed: 13139263 32644: Robbins FC, et al. Studies on the cultivation of poliomyelitis viruses in tissue culture. V. The direct isolation and serologic identification of virus strains in tissue culture from patients with nonparalytic and paralytic poliomyelitis. Am. J. Hyg. 54: 286-293, 1951. PubMed: 14877809