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ATCC? Number:VR-892?
Classification: Reoviridae, Rotavirus
Agent: Porcine rotavirus
Strain: OSU (virulent, gut origin)
Original Source: clinical specimen - animal (intestinal contents of one- to four-week-old pigs with diarrhea)Ohio, United States
Depositors: EH Bohl
Biosafety Level:2
Shipped: frozen
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Host Organism : Sus scrofa
Incubation : Atmosphere: 5% CO2 in air recommended Temperature: 36.0°C Duration: 4-7 days with some rocking; For best results cells should be 24 to 72 hours old and lightly 100% confluent. Pretreatment of virus with trypsin is needed to activate virus before inoculation.
[Note: Do not use serum in the culture medium.]
Effect : Yes, in vitro effects: Cytopathic effects (some rounding, cell degeneration and sloughing) with addition of pancreatin or trypsin at a level slightly under a toxic dose for the monolayer Yes, in vivo effects: diarrhea in small pigs
Store at : -70°C (or colder)
Comments : Serotype 5 (G5) by antigenic analysis of VP7 and serotype P7 by antigenic analysis of VP4. Isolated in gnotobiotic pigs. Preparation is of gut origin and its viral ancestry is of gut origin only.