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平臺編號：bio-49869
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國際編號：ATCC VR-893
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拉丁屬名： Porcine rotavirus
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規(guī)格：frozen
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用途：ATCC原裝進口
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
OSU (attenuated)
ATCC? Number:VR-893?
Classification: Reoviridae, Rotavirus
Agent: Porcine rotavirus
Strain: OSU (attenuated)
Original Source: clinical specimen - animal (intestinal contents of one- to four-week-old pigs with diarrhea)Ohio, United States
Depositors: LJ Saif
Biosafety Level:2
Shipped: frozen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Incubation : Temperature: 37.0°C Duration: 1-4 days; 5% CO2 in air atmosphere is recommended; For best results cells should be 24 to 48 hours old and 90-100% confluent. Protocol: This rotavirus should be grown in the presence of trypsin or pancreatin and NO serum should be used. Preliminary trials in gnotobiotic pigs indicate that this preparation is attenuated, but no back passages have been done. Cross-reacts with group A rotaviruses from other species. Heat labile (56擄C, 30min.). Stable at pH 3.0 and to ether.
Effect : Yes, in vitro effects: Cytopathic effects, some initial rounding followed by degeneration of cells and eventual sloughing
Store at : -70°C (or colder)
Comments : Serotype 5 (G5) by antigenic typing at VP7 and serotype P7 by antigenic typing at VP4. Preliminary trials in gnotobiotic pigs indicate that this preparation is attenuated.
References : 33985: Saif LJ, et al. Immune electron microscopy of transmissible gastroenteritis virus and rotavirus (reovirus-like agent) of swine. Am. J. Vet. Res. 38: 13-20, 1977. PubMed: 189646 33987: Theil KW, et al. Pathogenesis of porcine rotaviral infection in experimentally inoculated gnotobiotic pigs. Am. J. Vet. Res. 39: 213-220, 1978. PubMed: 204232 33989: Rosen BI, et al. Characterization of full-length and polymerase chain reaction-derived partial-length Gottfried and OSU gene 4 probes for serotypic differentiation of porcine rotaviruses. J. Clin. Microbiol. 30: 2644-2652, 1992. PubMed: 1328281 33990: Theil KW, et al. Cell culture propagation of porcine rotavirus (reovirus-like agent). Am. J. Vet. Res. 38: 1765-1768, 1977. PubMed: 201198
Classification: Reoviridae, Rotavirus
Agent: Porcine rotavirus
Strain: OSU (attenuated)
Original Source: clinical specimen - animal (intestinal contents of one- to four-week-old pigs with diarrhea)Ohio, United States
Depositors: LJ Saif
Biosafety Level:2
Shipped: frozen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Incubation : Temperature: 37.0°C Duration: 1-4 days; 5% CO2 in air atmosphere is recommended; For best results cells should be 24 to 48 hours old and 90-100% confluent. Protocol: This rotavirus should be grown in the presence of trypsin or pancreatin and NO serum should be used. Preliminary trials in gnotobiotic pigs indicate that this preparation is attenuated, but no back passages have been done. Cross-reacts with group A rotaviruses from other species. Heat labile (56擄C, 30min.). Stable at pH 3.0 and to ether.
Effect : Yes, in vitro effects: Cytopathic effects, some initial rounding followed by degeneration of cells and eventual sloughing
Store at : -70°C (or colder)
Comments : Serotype 5 (G5) by antigenic typing at VP7 and serotype P7 by antigenic typing at VP4. Preliminary trials in gnotobiotic pigs indicate that this preparation is attenuated.
References : 33985: Saif LJ, et al. Immune electron microscopy of transmissible gastroenteritis virus and rotavirus (reovirus-like agent) of swine. Am. J. Vet. Res. 38: 13-20, 1977. PubMed: 189646 33987: Theil KW, et al. Pathogenesis of porcine rotaviral infection in experimentally inoculated gnotobiotic pigs. Am. J. Vet. Res. 39: 213-220, 1978. PubMed: 204232 33989: Rosen BI, et al. Characterization of full-length and polymerase chain reaction-derived partial-length Gottfried and OSU gene 4 probes for serotypic differentiation of porcine rotaviruses. J. Clin. Microbiol. 30: 2644-2652, 1992. PubMed: 1328281 33990: Theil KW, et al. Cell culture propagation of porcine rotavirus (reovirus-like agent). Am. J. Vet. Res. 38: 1765-1768, 1977. PubMed: 201198
