Porcine parvovirus NADL-2 拉丁名
ATCC
VR-742
編號
Classification Parvoviridae, Parvovirus, Porcine parvovirus
Deposited As Porcine parvovirus
Agent Porcine parvovirus NADL-2
Strain NADL-2
Biosafety Level 安全等級 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments
At the time this deposit was made, TC of other species had not been extensively evaluated as viral hosts.
HA activity in chicken, rat, cat, and GP RBC at 22°C and 4°C (HA activity is four fold higher at 4°C than at 22°C for cat RBC). No HA activity in pig, hamster, sheep or cattle RBC. Virus must be freeze-thawed 3 times before inoculating and/or passing with fresh cells. CPE most pronounced when cells are exposed to the virus just before or after subculturing.
Effect on Host
CPE, nuclear inclusions and degeneration
Recommended Host
ST (ATCC CRL-1746)
Growth Conditions Temperature 37°C
Recommendations for Infection: This product is produced by co-cultivation of virus with fresh host cells. Prepare a bulk cell suspension the day of inoculation. Seed culture vessels at 30-50% confluency. Calculate the volume of virus needed to achieve an optimal MOI (e.g. 1:100 dilution) and then dilute virus in virus growth medium to prepare the virus inoculum. Add virus inoculum to culture vessels. Incubate for 24 hours at 37°C in a humidified 5% CO2 atmosphere. Aspirate virus growth medium to remove any traces of DMSO and then add fresh virus growth medium to cultures. Continue incubation.
Incubation: 3 -5 days
Effect on Host
CPE, nuclear inclusions and degeneration
Name of Depositor WL Mengeling
Source Porcine leukocytes from blood samples collected at an abattoir
References
Mengeling WL. Porcine parvovirus: properties and prevalence of a strain isolated in the United States. Am. J. Vet. Res. 33: 2239-2248, 1972. PubMed: 4628211
