ATCC? Number:VR-1374?
Classification: Poxviridae, Orthopoxvirus, Vaccinia virus
Agent: Ectromelia virus
Strain: Moscow
Original Source: tissue, animal (spleen homogenate from a BALB/c mouse 6 days post-infection)
Depositors: RM Buller
Biosafety Level:2
Shipped: frozen
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Host Organism : Mus musculus
Incubation : Atmosphere: 5% CO2 in air recommended Temperature: 37.0°C Duration: 1-7 days; For best results cells should be 24 to 48 hours old and 80-90% confluent (not 100% confluent).
Effect : Yes, in vitro effects: cytopathic effects, refractile rounding Yes, in vivo effects: death of mouse
Store at : -70°C (or colder)
Comments : Inadvertent introduction of Ectromelia virus into mouse colonies has caused major outbreaks in US animal colonies. The depositor recommends that animal care and biosafety management at your institution be consulted prior to initiating work with this agent. Requests for the agent must carry a signed statement assuming all risk and responsibility for handling and use. For additional information see: Fenner. 1982. Mousepox, (in) The Mouse in Biomedical Research: Vol. II. Diseases, (ed) Foster et al., Academic Press.