ATCC? Number:VR-118?
Classification: Poxviridae, Orthopoxvirus
Agent: Vaccinia virus deposited as Vaccinia virus, Orthopoxvirus
Strain: Lederle-Chorioallantoic
Original Source: clinical specimen - human (man, New York City Department of Health strain)
Depositors: PJ Vasington
Biosafety Level:2 [CDC BMBL recommends vaccination (Vaccination recommended for laboratory staff manipulating vaccinia, monkeypox, or cowpox viruses [CDC/NIH Biosafety in Microbiological and Biomedical Laboratories, 4th ed, 1999 - stock no. 017-040-00547-4].)]
Shipped: frozen
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Host Organism : chicken embryo, chorioallantoic membrane inoculation chicken embryo; chicken embryo; rabbit kidney cells; hamster kidney cells; KB cells (ATCCCCL-17); WI-38 cells (ATCCCCL-75); LLC-MK2 cells (ATCCCCL-7); Vero cells (ATCCCCL-81)
Incubation : Duration: 3-4 days
Effect : Yes, in vitro effects: Cytopathic effects in tissue culture Yes, in vivo effects: death of embryo Yes, in vivo effects: pocks on chorioallantoic membrane
Store at : -70°C (or colder)
Comments : The virus is very resistant and may contaminate the laboratory. The seed strain was used at Lederle Laboratories for preparation of smallpox calf lymph vaccine.