ATCC? Number:VR-299?
Classification: Birnaviridae,Aquabirnavirus
Agent: Infectious pancreatic necrosis virus
Strain: LWVRT 60-1
Original Source: Rainbow trout (Salmo gairdneri), West Virginia, 1960
Depositors: KE Wolf, MC Quimby
Biosafety Level:2
Shipped: frozen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Incubation : Temperature: 18.0°C Duration: 1 to 2 days; For best results cells should be 24 to 48 hours old and 70-80% confluent (not 100% confluent).
Effect : Yes, in vitro effects: cytopathic effect, refractile rounding
Comments : Brook trout 4 weeks of age become ill and die 1 to 4 weeks after feeding the virus. Antigenic for Salmo gairdneri, Salvelinus frontinalis, goats and rabbits. Replication of the virus has not been observed in amphibian or mammalian cell lines. This is a serious salmonid fish pathogen. It apparently originated in North America but has since been introduced to Europe and Japan. Stable at -20C and in 50% glycerol at 4C.
References : 24517: et al., Wolf K. . Virology 21: 317-321, 1963. 24533: Snieszko SF, et al. Virus nature of infectious pancreatic necrosis in trout. Proc. Soc. Exp. Biol. Med. 104: 105-108, 1960. PubMed: 13845654