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ATCC? Number:VR-2430?
Classification: Arteriviridae
Agent: Porcine respiratory and reproductive syndrome virus
Strain: ISU-55-SAH (plaque purified)
Original Source: pneumonic pig lungs, United States
Depositors: Iowa State Univ. Res. Fndn.
Biosafety Level:2
Shipped: frozen
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Incubation : Temperature: 37.0°C Duration: 2-3 days at 37C in PSP-36-SAH cells
Effect : Yes, in vitro effects: Cytopathic effects (degeneration, rounding, and clumping of cells)
Store at : -70°C (or colder)
Comments : This material is bacterially contaminated.This virus produces respiratory and reproductive disease in pigs. Strain is used for the production of vaccine against porcine respiratory and reproductive syndrome. Inoculate 2-3 day-old confluent monolayers with undiluted or 1:10 dilution of virus stock. After 1 hour add culture medium containing 2% FBS.
References : 38246: Paul PS, et al. Highly virulent porcine reproductive and respiratory syndrome viruses which produce lesions in pigs and vaccines that protect pigs against SAID syndrome. US Patent 5,695,766 dated Dec 9 1997