ATCC? Number:VR-2428?
Classification: Arteriviridae
Agent: Porcine respiratory and reproductive syndrome virus
Strain: ISU-51-SAH (plaque purified)
Original Source: pneumonic pig lungs, United States
Depositors: Iowa State Univ. Res. Fndn.
Biosafety Level:2
Shipped: frozen
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Host Organism : PSP-36 cells (ATCCCRL-11171) PSP-36-SAH cells; pig
Incubation : Temperature: 37.0°C Duration: 2-3 days at 37C in PSP-36 cells (ATCCCRL-11171)
Effect : Yes, in vitro effects: Cytopathic effects (degeneration, rounding, and clumping of cells) Yes, in vitro effects: Presence of virus can be confirmed with conjugated monoclonal antibody.
Store at : -70C
Comments : This virus produces respiratory and reproductive disease in pigs. Strain is used for the production of vaccine against porcine respiratory and reproductive syndrome. -70C Inoculate 2-3 day-old confluent monolayers with undiluted or 1:10 dilution of virus stock. After 1 hour add culture medium containing 2% FBS.