ATCC? Number:VR-585?
Classification: Herpesviridae, Mardivirus
Agent: Gallid herpesvirus 2 deposited as Marek's disease virus type 1
Strain: JM
Original Source: chicken tumor; blood
Depositors: RL Witter
Biosafety Level:2
Shipped: frozen
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Host Organism : chicken, i.p. inoculation; chicken kidney cells; duck embryo fibroblasts chicken, i.p. inoculation; chorioallantoic membrane; chicken kidney cells; duck embryo fibroblasts
Incubation : Temperature: 37.0°C Duration: 7-14 days
Effect : Yes, in vitro effects: Cytopathic effects (herpesvirus-like CPE) in tissue culture Yes, in vivo effects: Marek's disease lesions in chickens Yes, in vivo effects: pocks on chorioallantoic membrane in eggs
Comments : Strongly cell associated in vitro and in vivo except for feather follicle epithelium of infected chickens. Stable for at least 2-3 years in viable cells frozen at -196C. Strongly cell associated in vitro and in vivo except for feather follicle epithelium of infected chickens.
References : 33672: Sevoian M. . Vet. Med. 57: 500, 1962. 33673: Solomon JJ, et al. Studies on the etiology of Marek's disease. I. Propagation of the agent in cell culture. Proc. Soc. Exp. Biol. Med. 127: 173-177, 1968. PubMed: 5644639 33674: Stephens EA, et al. Characteristics of JMV Marek's disease tumor: a nonproductively infected transplantable cell lacking in rescuable Virus. J. Natl. Cancer Inst. 57: 865-874, 1976. PubMed: 187779