ATCC? Number:VR-3131?
Classification: Poxviridae, Orthopoxvirus
Agent: Vaccinia virus ts mutant
Strain: IHD-W Dts9
Depositors: S Dales, R. Condit
Biosafety Level:2 [BSL 2 Vaccination is recommended for laboratory staff working with vaccinia, monkeypox and cowpox viruses ]
Shipped: frozen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Host Organism : Production Host: BSC-40 (ATCCCRL-2761) Alternate Host(s): MRC-5, BS-C-1, HeLa, LLC-MK2
Incubation : 1-3 days at 31 C with 5% CO2
Effect : Cytopathic effects in culture include enlargement and rounding of cells, followed by death and sloughing
Store at : -70 C or lower
Comments : Selected after mutagenesis with nitrosoguanidine. The non-permissive incubation temperature is 39.5 C. This mutant was assigned to Dales EM category E, inducing foci of viroplasm, aberrant membranes without spicules, and DNA paracrystals in infected cells. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location D13, which encodes a 65-kDa protein thought to function as a scaffold protein for the formation of viral crescents and immature virions. [70405]
Related Products: VR-1441 Vaccinia virus, strain IHD-W