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平臺編號：bio-50138
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國際編號：ATCC VR-3151
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拉丁屬名： Vaccinia virus ts mutant
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規(guī)格：frozen
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用途：ATCC原裝進口
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服務費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產品信息以出庫為準）
中文名稱暫無
ATCC? Number:VR-3151?
Classification: Poxviridae, Orthopoxvirus
Agent: Vaccinia virus ts mutant
Strain: IHD-W Dts56
Depositors: S. Dales, R. Condit
Biosafety Level:2 [BSL 2 ]
Shipped: frozen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Host Organism : Production Host: BSC-40 (ATCCCRL-2761) Alternate Host(s): MRC-5, BS-C-1, HeLa, LLC-MK2
Incubation : 1-3 days at 31 C with 5% CO2
Effect : Cytopathic effects in culture include cell enlargement and rounding, followed by death and sloughing
Store at : -70 C or colder
Comments : This vaccinia mutant, originally designated plaque isolate no. 7473, was isolated from the IHD-W strain of Vaccinia by S. Dales, presumably by mutagenesis with nitrosoguanidine followed by replication in the presence of bromodeoxyuridine. It was not assigned to a Dales EM category (Dales, et al, 2003), but was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location D5, an ATPase required for DNA replication. Shifting infection conditions from permissive to non-permissive temperature (39.5 C) results in an abrupt cessation of DNA synthesis, described as a fast stop phenotype.Vaccination is recommended for laboratory staff manipulating vaccinia, monkeypox and cowpox viruses. [33180] [70405]
Related Products: VR-1441 Vaccinia virus, strain IHD-W
Classification: Poxviridae, Orthopoxvirus
Agent: Vaccinia virus ts mutant
Strain: IHD-W Dts56
Depositors: S. Dales, R. Condit
Biosafety Level:2 [BSL 2 ]
Shipped: frozen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Host Organism : Production Host: BSC-40 (ATCCCRL-2761) Alternate Host(s): MRC-5, BS-C-1, HeLa, LLC-MK2
Incubation : 1-3 days at 31 C with 5% CO2
Effect : Cytopathic effects in culture include cell enlargement and rounding, followed by death and sloughing
Store at : -70 C or colder
Comments : This vaccinia mutant, originally designated plaque isolate no. 7473, was isolated from the IHD-W strain of Vaccinia by S. Dales, presumably by mutagenesis with nitrosoguanidine followed by replication in the presence of bromodeoxyuridine. It was not assigned to a Dales EM category (Dales, et al, 2003), but was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location D5, an ATPase required for DNA replication. Shifting infection conditions from permissive to non-permissive temperature (39.5 C) results in an abrupt cessation of DNA synthesis, described as a fast stop phenotype.Vaccination is recommended for laboratory staff manipulating vaccinia, monkeypox and cowpox viruses. [33180] [70405]
Related Products: VR-1441 Vaccinia virus, strain IHD-W
