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ATCC? Number:VR-3153?
Classification: Poxviridae, Orthopoxvirus
Agent: Vaccinia virus ts mutant
Strain: IHD-W Dts66
Depositors: S. Dales, R. Condit
Biosafety Level:2
Shipped: frozen
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Host Organism : Production host:  BSC-40 (ATCCCRL-2761) Alternate hosts:   MRC-5, BSC-1, HeLa, LLC-MK2
Incubation : For best results, plate cells 24-48 hours in advance in DMEM with 10% FBS at 37.0°C.  Infect at 90-100% confluence using a multiplicity of infection (MOI) of about 1.  Infect and incubate in DMEM supplemented with 2% FBS at 31.0°C for 1-3 days.  Harvest cells and culture fluids when 100% of culture shows advanced cytopathic effects.  Vaccinia virus is largely cell associated.  Concentrated preparations can be made by collection and sonication of cell pellets.
Effect : Cytopathic effects in culture include enlargement and rounding of cells, followed by death and sloughing.
Store at : -70 C or colder
Comments : This conditional lethal mutant, originally designated plaque isolate no. 7954, was isolated from the IHD-W strain of Vaccinia by S. Dale after mutagenesis with nitrosoguanidine and replication in the presence of bromodeoxyuridine (Dales, et al, 1978).  The non-permissive incubation temperature is 39.5°C.  The mutant was not assigned to a Dales EM category. Its mutation was assigned by complementation analysis (Lackner, et al, 2003) to map location A24, the 132-kDa viral RNA polymerase subunit. Vaccination is recommended for laboratory staff manipulating vaccinia, monkeypox, and cowpox viruses.
Related Products: Virus: ATCC VR-1441