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平臺編號：bio-50143
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國際編號：ATCC VR-3121
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拉丁屬名： Vaccinia virus ts mutant
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規(guī)格：frozen
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用途：ATCC原裝進口
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服務費用：
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注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
中文名稱暫無
ATCC? Number:VR-3121?
Classification: Poxviridae, Orthopoxvirus
Agent: Vaccinia virus ts mutant
Strain: IHD-W Dts8 (Dales isolate 991)
Original Source: derived from existing strain (This mutant strain was derived by Samuel Dales from the IHD-W strain of Vaccinia (ATCCVR-1441) which in turn was derived from the IHD-J strain.)
Depositors: S Dales, RC Condit, S Dales
Biosafety Level:2
Shipped: frozen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tested Positive for Mycoplasma : No
Incubation : Temperature: 31.0°C Duration: 1-3 days; For best results, cells should be 24 to 48 hours old and 80-90% confluent at time of infection [not 100% confluent].
Effect : Yes, Cytopathic effect: Rounding and sloughing of infected cells
Comments : Selected after mutagenesis with nitrosoguanidine. The non-permissive incubation temperature is 39.5oC. This mutant was assigned to Dales EM category L indicating that it produces immature particles with nucleoids and defective membranes with spicules. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location U5.
References : 70405: Lackner CA, et al. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305: 240-259, 2003. PubMed: 12573570
Classification: Poxviridae, Orthopoxvirus
Agent: Vaccinia virus ts mutant
Strain: IHD-W Dts8 (Dales isolate 991)
Original Source: derived from existing strain (This mutant strain was derived by Samuel Dales from the IHD-W strain of Vaccinia (ATCCVR-1441) which in turn was derived from the IHD-J strain.)
Depositors: S Dales, RC Condit, S Dales
Biosafety Level:2
Shipped: frozen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tested Positive for Mycoplasma : No
Incubation : Temperature: 31.0°C Duration: 1-3 days; For best results, cells should be 24 to 48 hours old and 80-90% confluent at time of infection [not 100% confluent].
Effect : Yes, Cytopathic effect: Rounding and sloughing of infected cells
Comments : Selected after mutagenesis with nitrosoguanidine. The non-permissive incubation temperature is 39.5oC. This mutant was assigned to Dales EM category L indicating that it produces immature particles with nucleoids and defective membranes with spicules. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location U5.
References : 70405: Lackner CA, et al. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305: 240-259, 2003. PubMed: 12573570
