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平臺編號：bio-50145
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國際編號：ATCC VR-3148
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拉丁屬名： Vaccinia virus ts mutant
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規(guī)格：frozen
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用途：ATCC原裝進口
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服務費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產品信息以出庫為準）
中文名稱暫無
ATCC? Number:VR-3148?
Classification: Poxviridae, Orthopoxvirus
Agent: Vaccinia virus ts mutant
Strain: IHD-W Dts40 (Dales isolate 3148)
Original Source: derived from existing strain (This mutant strain was derived by Samuel Dales from the IHD-W strain of Vaccinia (ATCCVR-1441) which in turn was derived from the IHD-J strain.)
Depositors: S Dales, RC Condit, S Dales
Biosafety Level:2
Shipped: frozen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tested Positive for Mycoplasma : No
Incubation : Temperature: 31.0°C Duration: 1-3 days; For best results, cells should be 24 to 48 hours old and 80-90% confluent at time of infection [not 100% confluent].
Effect : Yes, Cytopathic effect: Rounding and sloughing of infected cells
Comments : Selected after mutagenesis with nitrosoguanidine and replication in the presence of bromodeoxyuridine. The non-permissive incubation temperature is 39.5oC. This mutant was assigned to Dales EM category P, characterized by immature normal particles and mature particles with cores that are predominantly lucent at the center. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location U11.
References : 33180: Dales S, et al. Biogenesis of vaccinia: isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84: 403-428, 1978. PubMed: 622807 61241: Condit RC, et al. Isolation, characterization, and physical mapping of temperature-sensitive mutants of vaccinia virus. Virology 128: 429-443, 1983. PubMed: 6577746 61305: . Biosafety in Microbiological and Biomedical Laboratories 4th ed.. : ; 1999. 70405: Lackner CA, et al. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305: 240-259, 2003. PubMed: 12573570
Classification: Poxviridae, Orthopoxvirus
Agent: Vaccinia virus ts mutant
Strain: IHD-W Dts40 (Dales isolate 3148)
Original Source: derived from existing strain (This mutant strain was derived by Samuel Dales from the IHD-W strain of Vaccinia (ATCCVR-1441) which in turn was derived from the IHD-J strain.)
Depositors: S Dales, RC Condit, S Dales
Biosafety Level:2
Shipped: frozen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tested Positive for Mycoplasma : No
Incubation : Temperature: 31.0°C Duration: 1-3 days; For best results, cells should be 24 to 48 hours old and 80-90% confluent at time of infection [not 100% confluent].
Effect : Yes, Cytopathic effect: Rounding and sloughing of infected cells
Comments : Selected after mutagenesis with nitrosoguanidine and replication in the presence of bromodeoxyuridine. The non-permissive incubation temperature is 39.5oC. This mutant was assigned to Dales EM category P, characterized by immature normal particles and mature particles with cores that are predominantly lucent at the center. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location U11.
References : 33180: Dales S, et al. Biogenesis of vaccinia: isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84: 403-428, 1978. PubMed: 622807 61241: Condit RC, et al. Isolation, characterization, and physical mapping of temperature-sensitive mutants of vaccinia virus. Virology 128: 429-443, 1983. PubMed: 6577746 61305: . Biosafety in Microbiological and Biomedical Laboratories 4th ed.. : ; 1999. 70405: Lackner CA, et al. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305: 240-259, 2003. PubMed: 12573570
